
플라스미드 매개 항균 저항성 전파에 관여하는 면역글로불린 유사 도메인을 포함하는 단백질을 표적화하여 항균 저항성을 극복할 수 있다.


coremsg

Antimicrobial resistance (AMR) poses a significant threat to human health. Although vaccines have been developed to combat AMR, it has proven challenging to associate specific vaccine antigens with AMR. Bacterial plasmids play a crucial role in the transmission of AMR. Our recent research has identified a group of bacterial plasmids (specifically, IncHI plasmids) that encode large molecular mass proteins containing bacterial immunoglobulin-like domains. These proteins are found on the external surface of the bacterial cells, such as in the flagella or conjugative pili. In this study, we show that these proteins are antigenic and can protect mice from infection caused by an AMR Salmonella strain harboring one of these plasmids. Furthermore, we successfully generated nanobodies targeting these proteins, that were shown to interfere with the conjugative transfer of IncHI plasmids. Considering that these proteins are also encoded in other groups of plasmids, such as IncA/C and IncP2, targeting them could be a valuable strategy in combating AMR infections caused by bacteria harboring different groups of AMR plasmids. Since the selected antigens are directly linked to AMR itself, the protective effect extends beyond specific microorganisms to include all those carrying the corresponding resistance plasmids.

### Competing Interest Statement

The authors have declared no competing interest.

Targeting plasmid-encoded proteins that contain immunoglobulin-like domains to combat antimicrobial resistance

항균-저항성-극복을-위한-면역글로불린-유사-도메인을-포함하는-플라스미드-암호화-단백질-표적화


항균 저항성 극복을 위한 면역글로불린 유사 도메인을 포함하는 플라스미드 암호화 단백질 표적화
