NLRP3 S-acylation Mechanism for Golgi Access Control

מושגי ליבה

S-acylation of NLRP3 at Cys-130 controls Golgi access through a dynamic cycle.

תקציר

• NLRP3 is an inflammasome receptor activated by danger signals.
• Electrostatic interactions and hydrophobic residues drive NLRP3 to the Golgi.
• S-acylation at Cys-130 crucial for NLRP3 membrane association.
• Nigericin disrupts Golgi trafficking, leading to NLRP3 immobilization.
• ZDHHC enzymes influence NLRP3 localization through S-acylation.
• APT2 thioesterase regulates NLRP3 de-acylation and Golgi binding.
• Impaired trafficking in the Golgi limits APT2-NLRP3 interaction post-nigericin.

סטטיסטיקה

S-Acylation of proteins can be reversed by thioesterase enzymes.
Small molecule inhibition of thioesterase activity affects protein localization.

ציטוטים

"Nigericin treatment suppressed the fluorescence recovery, indicating less mobile NLRP3."
"Enhanced recruitment of NLRP3 to the Golgi correlated with increased S-acylated species."

תובנות מפתח מזוקקות מ:

S-acylation of NLRP3 provides a nigericin sensitive gating mechanism that controls access to the Golgi

by Williams,D. ... ב- www.biorxiv.org 11-14-2023

https://www.biorxiv.org/content/10.1101/2023.11.14.566891v3

שאלות מעמיקות

How does the dynamic S-acylation cycle impact NLRP3 function beyond Golgi localization?

The dynamic S-acylation cycle of NLRP3 plays a crucial role in regulating its function beyond just Golgi localization. S-acylation at Cys-130 is essential for stabilizing NLRP3 on the Golgi membrane, but it also influences the activation and signaling pathways of NLRP3. The reversible nature of S-acylation allows for rapid changes in protein localization and activity in response to cellular stimuli.
One significant impact of the S-acylation cycle on NLRP3 function is its involvement in inflammasome activation. Proper recruitment of NLRP3 to intracellular membranes, facilitated by S-acylation, is necessary for the formation and activation of the inflammasome complex. Disruption or dysregulation of this process can lead to aberrant inflammasome activation, contributing to inflammatory diseases.
Moreover, post-translational modifications such as phosphorylation or ubiquitination may interact with S-acylation to modulate NLRP3 activity and downstream signaling pathways. For example, phosphorylation events could regulate the accessibility or affinity of ZDHHC enzymes responsible for acylating NLRP3 at Cys-130.
In summary, the dynamic S-acylation cycle not only controls Golgi localization but also impacts various aspects of NLRP3 function including inflammasome assembly, signal transduction processes, and overall immune responses.

How might other post-translational modifications interact with S-acylation to regulate NLRP3?

Other post-translational modifications can indeed interact with S-acylations like those occurring at Cys 130 withinNRLRPs structureto fine-tune its regulatory mechanisms:

Phosphorylations: Phosphorylations are known to influence protein-protein interactions and subcellular localizations; they could potentially affect either positively or negatively how NRLRP interacts with ZDHHCs that performS-AcyaltiononCysteine residues.

Ubiquitinations: Ubiquitinations often mark proteins for degradation via proteasomes; thus,ZDHHC-mediatedS-Acyaltionscould be regulated through ubiquitination-dependent turnover mechanisms impactingNRLRP's stabilityand functionality.

These interactions between different typesofpost-translationalmodificationscan create a sophisticated networkof regulationforNRLRPfunctionalitybeyondjustGolgilocalization,making ita highly adaptableproteininresponse todynamiccellularstimuli.

How might understandingtheintracellular trafficking dynamics of proteins like NRLRP contribute todisease treatmentor drug development?

Understandingtheintracellular traffickingdynamicsprovides valuable insights into disease pathogenesisand offers potential therapeutic targetsfor intervention:
1.Inhibiting specific PATsenzymes involvedinS-Acyaltionprocessescouldbeexploredas a strategytoregulatetheactivityofNRLRPand mitigateexcessive inflammationassociatedwith certain diseaseslikeatherosclerosisorAlzheimer'sdisease.
2.TargetingthioesteraseenzymeslikeAPT2thatde-Acylateproteinscouldofferanapproachto modulateproteinlocalizationandsignalingevents,suchasinflammasomeregulationbycontrollingthebalancebetweenAcylatedandDe-AcylatedstatesofproteinsliketheNRLRPcomplex
By elucidatinghowtheseintracellulartraffickingmechanismsimpactproteinfunctionality,diseasetreatmentstrategiescanbedevelopedtotargetspecificstepsintheprocessesinvolvedinpathologicalconditionslinkedtoaberrantproteinlocalizationorsignalingactivities,revealingnoveltherapeuticopportunitiesforthetreatmentofvariousdiseasesincludingthoseimplicatingthenucleotide-bindingdomainleucinerichrepeatfamily,presentingnewavenuesfordrugdevelopmentandresearchintoinflammatorydisordersandinflammasomedysregulationschemes