통찰 - Computational Biology - # Spatial and Functional Organization of the Human Genome

Comprehensive Analysis of Nuclear Genome Organization and Function Across Multiple Nuclear Locales in Human Cells

Q: How do the spatial and functional relationships between nuclear locales and genomic regions evolve during different cellular processes, such as differentiation or disease progression

The spatial and functional relationships between nuclear locales and genomic regions undergo dynamic changes during different cellular processes such as differentiation or disease progression. In the context of differentiation, as cells transition from a pluripotent state to more specialized cell types, there is a reorganization of nuclear architecture to support the specific gene expression programs required for the new cell fate. This reorganization involves changes in the positioning of genomic regions relative to nuclear bodies like the nuclear lamina, speckles, and nucleoli. For example, during differentiation, certain genes may relocate closer to nuclear speckles, which are associated with active transcription, leading to their upregulation. Conversely, genes that need to be repressed may move towards the nuclear lamina, a region linked to gene silencing. This spatial repositioning of genomic regions in relation to nuclear locales plays a crucial role in regulating gene expression patterns during differentiation. In the context of disease progression, alterations in nuclear organization can have profound effects on genome function. For instance, changes in the attachment of genomic regions to the nuclear lamina or other nuclear bodies can impact gene expression, DNA replication timing, and overall genome stability. In diseases such as cancer, aberrant nuclear organization can lead to dysregulated gene expression and genomic instability, contributing to disease progression. Understanding how the spatial and functional relationships between nuclear locales and genomic regions evolve during different cellular processes is essential for unraveling the molecular mechanisms underlying these processes and developing targeted therapeutic interventions.

Q: What are the underlying mechanisms that govern the dynamic competition between LADs and iLADs for attachment to the nuclear lamina, and how do these mechanisms impact genome function

The dynamic competition between Lamina-Associated Domains (LADs) and interstitial LADs (iLADs) for attachment to the nuclear lamina is governed by several underlying mechanisms. LADs are genomic regions that interact with the nuclear lamina and are often associated with gene silencing, while iLADs are partially repressed regions located in the nuclear interior. The competition between LADs and iLADs for lamina attachment is influenced by factors such as the binding affinity of specific proteins to the nuclear lamina, the epigenetic modifications present on these genomic regions, and the overall nuclear architecture. One key mechanism that impacts the competition between LADs and iLADs is the presence of specific proteins, such as Lamin A and LBR, which mediate the attachment of genomic regions to the nuclear lamina. Knockout experiments have shown that the loss of these proteins can lead to a shift in the localization of LADs and iLADs within the nucleus, affecting their replication timing and gene expression patterns. Additionally, the epigenetic modifications present on LADs and iLADs, such as H3K9me3 and H3K27me3, play a role in determining their affinity for the nuclear lamina and their functional state. The competition between LADs and iLADs for attachment to the nuclear lamina has significant implications for genome function. Changes in the localization of these genomic regions can impact DNA replication timing, gene expression, and overall nuclear organization. Understanding the mechanisms that govern this competition is crucial for deciphering how nuclear architecture influences genome function and cellular processes.

Q: What are the broader implications of the polarized nuclear organization observed in adherent cells, and how might this organization be altered in different cell types or physiological conditions

The polarized nuclear organization observed in adherent cells has broader implications for genome organization and function. In adherent cells, nuclear bodies and genomic regions segregate both radially and relative to the equatorial plane, creating a spatially organized nuclear architecture. This polarization of nuclear organization is essential for coordinating gene expression, DNA replication, and other nuclear processes in a spatially regulated manner. The polarized nuclear organization in adherent cells may be altered in different cell types or physiological conditions, leading to changes in genome function. For example, in disease states or during cellular stress, the nuclear organization may become disrupted, affecting gene expression patterns and genomic stability. Changes in the polarization of nuclear bodies and genomic regions can impact the efficiency of DNA replication, the regulation of gene expression, and the overall functional organization of the genome. Understanding how the polarized nuclear organization is maintained or altered in different cell types or physiological conditions is crucial for elucidating the impact of nuclear architecture on genome function. By studying the dynamics of nuclear organization in various cellular contexts, researchers can gain insights into the mechanisms that govern genome organization and function, providing valuable information for both basic research and potential therapeutic interventions.

핵심 개념

Nuclear genome organization is defined by multiple nuclear locales, including the nuclear lamina, nuclear speckles, and nucleoli, beyond the binary active/inactive compartment model.

초록

The article presents a comprehensive analysis of nuclear genome organization and function in human cells, integrating sequencing and image-based data. The key findings are:

Gene expression correlates mostly with proximity to nuclear speckles, while DNA replication timing correlates with proximity to multiple nuclear locales.
Speckle attachment regions emerge as DNA replication initiation zones, with their replication timing and gene composition varying based on attachment frequency.
Most facultative lamina-associated domains (LADs) retain a partially repressed state as interior LADs (iLADs), despite their positioning in the nuclear interior.
Knockout of lamina proteins Lamin A and LBR causes a shift of H3K9me3-enriched LADs from the lamina to the nucleolus, and a reciprocal relocation of H3K27me3-enriched partially repressed iLADs from the nucleolus to the lamina.
The nuclear organization in adherent cells is polarized, with nuclear bodies and genomic regions segregating both radially and relative to the equatorial plane.

The study highlights the importance of considering genome organization relative to multiple nuclear locales, beyond the binary active/inactive compartment model, for a more comprehensive understanding of the spatial and functional organization of the human genome.

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biorxiv.org

통계

Gene expression correlates mostly with proximity to nuclear speckles.
DNA replication timing correlates with proximity to multiple nuclear locales.
Speckle attachment regions are DNA replication initiation zones.
Replication timing and gene composition of speckle attachment regions vary with attachment frequency.
Knockout of Lamin A and LBR causes a shift of H3K9me3-enriched LADs from lamina to nucleolus, and a reciprocal relocation of H3K27me3-enriched iLADs from nucleolus to lamina.

인용구

"Whereas gene expression correlates mostly with nuclear speckle proximity, DNA replication timing correlates with proximity to multiple nuclear locales."
"Speckle attachment regions emerge as DNA replication initiation zones whose replication timing and gene composition vary with their attachment frequency."
"Most facultative LADs retain a partially repressed state as iLADs, despite their positioning in the nuclear interior."

핵심 통찰 요약

Beyond A and B Compartments: how major nuclear locales define nuclear genome organization and function

by Gholamalamda... 게시일 www.biorxiv.org 04-23-2024

https://www.biorxiv.org/content/10.1101/2024.04.23.590809v2

더 깊은 질문

How do the spatial and functional relationships between nuclear locales and genomic regions evolve during different cellular processes, such as differentiation or disease progression

The spatial and functional relationships between nuclear locales and genomic regions undergo dynamic changes during different cellular processes such as differentiation or disease progression. In the context of differentiation, as cells transition from a pluripotent state to more specialized cell types, there is a reorganization of nuclear architecture to support the specific gene expression programs required for the new cell fate. This reorganization involves changes in the positioning of genomic regions relative to nuclear bodies like the nuclear lamina, speckles, and nucleoli. For example, during differentiation, certain genes may relocate closer to nuclear speckles, which are associated with active transcription, leading to their upregulation. Conversely, genes that need to be repressed may move towards the nuclear lamina, a region linked to gene silencing. This spatial repositioning of genomic regions in relation to nuclear locales plays a crucial role in regulating gene expression patterns during differentiation.
In the context of disease progression, alterations in nuclear organization can have profound effects on genome function. For instance, changes in the attachment of genomic regions to the nuclear lamina or other nuclear bodies can impact gene expression, DNA replication timing, and overall genome stability. In diseases such as cancer, aberrant nuclear organization can lead to dysregulated gene expression and genomic instability, contributing to disease progression. Understanding how the spatial and functional relationships between nuclear locales and genomic regions evolve during different cellular processes is essential for unraveling the molecular mechanisms underlying these processes and developing targeted therapeutic interventions.

What are the underlying mechanisms that govern the dynamic competition between LADs and iLADs for attachment to the nuclear lamina, and how do these mechanisms impact genome function

The dynamic competition between Lamina-Associated Domains (LADs) and interstitial LADs (iLADs) for attachment to the nuclear lamina is governed by several underlying mechanisms. LADs are genomic regions that interact with the nuclear lamina and are often associated with gene silencing, while iLADs are partially repressed regions located in the nuclear interior. The competition between LADs and iLADs for lamina attachment is influenced by factors such as the binding affinity of specific proteins to the nuclear lamina, the epigenetic modifications present on these genomic regions, and the overall nuclear architecture.
One key mechanism that impacts the competition between LADs and iLADs is the presence of specific proteins, such as Lamin A and LBR, which mediate the attachment of genomic regions to the nuclear lamina. Knockout experiments have shown that the loss of these proteins can lead to a shift in the localization of LADs and iLADs within the nucleus, affecting their replication timing and gene expression patterns. Additionally, the epigenetic modifications present on LADs and iLADs, such as H3K9me3 and H3K27me3, play a role in determining their affinity for the nuclear lamina and their functional state.
The competition between LADs and iLADs for attachment to the nuclear lamina has significant implications for genome function. Changes in the localization of these genomic regions can impact DNA replication timing, gene expression, and overall nuclear organization. Understanding the mechanisms that govern this competition is crucial for deciphering how nuclear architecture influences genome function and cellular processes.

What are the broader implications of the polarized nuclear organization observed in adherent cells, and how might this organization be altered in different cell types or physiological conditions

The polarized nuclear organization observed in adherent cells has broader implications for genome organization and function. In adherent cells, nuclear bodies and genomic regions segregate both radially and relative to the equatorial plane, creating a spatially organized nuclear architecture. This polarization of nuclear organization is essential for coordinating gene expression, DNA replication, and other nuclear processes in a spatially regulated manner.
The polarized nuclear organization in adherent cells may be altered in different cell types or physiological conditions, leading to changes in genome function. For example, in disease states or during cellular stress, the nuclear organization may become disrupted, affecting gene expression patterns and genomic stability. Changes in the polarization of nuclear bodies and genomic regions can impact the efficiency of DNA replication, the regulation of gene expression, and the overall functional organization of the genome.
Understanding how the polarized nuclear organization is maintained or altered in different cell types or physiological conditions is crucial for elucidating the impact of nuclear architecture on genome function. By studying the dynamics of nuclear organization in various cellular contexts, researchers can gain insights into the mechanisms that govern genome organization and function, providing valuable information for both basic research and potential therapeutic interventions.