
Anterior endoderm cells originate from prechordal plate progenitors, and their segregation is regulated by a balance of Nodal signaling, chromatin remodeling, and the collaborative actions of transcriptional regulators Gsc and Ripply1.


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During gastrulation, the mesendoderm is firstly specified by morphogens such as Nodal, and then segregates into endoderm and mesoderm in a Nodal concentration-dependent manner. However, the mechanism underlying the segregation and crosstalk of different sub-groups within the meso- and endoderm lineages remains unclear. Here, taking zebrafish prechordal plate (PP) and anterior endoderm (Endo) as research model, using single-cell multi-omics and live imaging analyses, we show that anterior Endo progenitors originate directly from PP progenitors. A single-cell transcriptomic trajectory analysis of wild-type, ndr1 knockdown and lft1 knockout Nodal explants confirms the diversification of anterior Endo fate from PP progenitors. Gene Ontology (GO) enrichment analysis indentifies that the change of chromatin organization potentiates the segregation of endodermal cell fate from PP progenitors. Single-cell ATAC & RNA sequencing further reveals that two transcriptional regulators, gsc and ripply1 , exhibit varied activation patterns in PP and Endo lineages at both the chromatin and RNA expression levels. We further demonstrate that Ripply1 functions coordinately with Gsc to repress endodermal cell fate by directly binding to the cis -elements of sox32 and sox17 . Modulating the expression levels of these regulators tilts the cell fate decision between the PP and Endo lineages.

### Competing Interest Statement

The authors have declared no competing interest.

Ripply1 and Gsc collectively suppress anterior endoderm differentiation from prechordal plate progenitors

dissecting-the-segregation-of-anterior-mesendoderm-lineages-at-the-single-cell-level-in-zebrafish


Dissecting the Segregation of Anterior Mesendoderm Lineages at the Single-Cell Level in Zebrafish
