แนวคิดหลัก
Maternal Caspar protein is essential for proper centrosome behavior, cytoskeletal organization, and primordial germ cell specification during early Drosophila embryonic development.
บทคัดย่อ
The content explores the maternal role of the Caspar (Casp) protein, the Drosophila ortholog of human Fas-associated factor-1 (FAF1), during early embryonic development in Drosophila melanogaster.
Key highlights:
- Maternal loss of Casp or its interacting partner TER94 leads to partial embryonic lethality, correlated with aberrant centrosome behavior, cytoskeletal abnormalities, and defective gastrulation.
- Casp and TER94 are enriched in the primordial germ cells (PGCs), and their maternal depletion results in a significant reduction in the PGC count.
- The total number of pole buds is directly proportional to the level of Casp, with its 'loss' and 'gain' resulting in respective reduction and increase in the Oskar protein levels, the master determinant of PGC fate.
- Casp regulates the degradation of the translational repressor Smaug, a zygotic regulator of germ cell specification, during the maternal-to-zygotic transition.
- Structure-function analysis of Casp domains reveals their distinct roles in regulating PGC number and division.
The study presents a detailed analysis of the novel involvement of the maternally provided Casp protein in PGC development and early embryonic patterning in Drosophila.
สถิติ
Maternal loss of Casp or TER94 leads to ~70% and ~95% embryonic lethality, respectively.
Maternal overexpression of Casp increases the total number of pole buds and PGCs by ~2-fold compared to control.
Maternal depletion of Casp results in a ~60% reduction in the total number of PGCs.
คำพูด
"Maternal loss of either Casp or it's protein partner, Transitional endoplasmic reticulum 94 (TER94) leads to partial embryonic lethality correlated with aberrant centrosome behavior, cytoskeletal abnormalities, and defective gastrulation."
"The total number of pole buds is directly proportional to the level of Casp, with its 'loss' and 'gain' resulting in respective reduction and increase in the Oskar protein levels, the master determinant of PGC fate."
"Casp regulates the degradation of the translational repressor Smaug, a zygotic regulator of germ cell specification, during the maternal-to-zygotic transition."