The study investigated the involvement of the temperature-sensitive ion channel TRPV4 in the regulation of perspiration in mice. The researchers found that TRPV4 is expressed and functionally active in mouse sweat gland secretory cells, where it colocalizes with the calcium-activated chloride channel ANO1 and the water channel AQP5.
Experiments showed that stimulated and basal sweating in mouse hind paws was temperature-dependent, with increased sweating at higher temperatures in wild-type mice but not in TRPV4-deficient mice. Menthol, which inhibits both TRPV4 and ANO1, reduced sweating in both wild-type and TRPM8-deficient mice, suggesting that the inhibition of TRPV4 and ANO1 contributes to the cooling sensation of menthol. Furthermore, the ANO1 inhibitor Ani9 almost completely abolished basal sweating, indicating a pivotal role of ANO1 in the sweating process.
The researchers also found that TRPV4 expression was significantly higher in normohidrotic skin from patients with acquired idiopathic generalized anhidrosis (AIGA) compared to anhidrotic skin from the same patients, suggesting that TRPV4 plays an important role in human perspiration as well. The TRPV4-ANO1 complex could be a target for developing agents to regulate perspiration and treat sweating disorders.
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by Kashio,M., D... о www.biorxiv.org 10-29-2023
https://www.biorxiv.org/content/10.1101/2023.10.25.563940v2Глибші Запити