
The nuclear basket proteins Mlp1, Mlp2, and Pml39 form a stable assembly on nuclear pore complexes (NPCs) and mediate their exclusion from the nucleolar territory, while Nup2 independently restricts the localization of NPCs to the non-nucleolar region.


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Nuclear pore complexes (NPCs) mediate all traffic between the nucleus and the cytoplasm and are among the most stable protein assemblies in cells. Intriguingly, budding yeast cells carry two variants of NPCs which differ in the presence or absence of the nuclear basket proteins Mlp1, Mlp2 and Pml39. The binding of these basket proteins ocurs very late in NPC assembly and Mlp-positive NPCs are excluded from the region of the nuclear envelope that borders the nucleolus.

Here, we use recombination-induced tag exchange (RITE) to investigate the stability of all the NPC subcomplexes within individual NPCs. We show that the nuclear basket proteins Mlp1, Mlp2 and Pml39 remain stably associated with NPCs through multiple cell-division cycles, and that Mlp1/2 are responsible for the exclusion of NPCs from the nucleolar territory. In addition, we demonstrate that binding of Nup2 also depletes Mlp-negative NPCs from this region by an independent pathway. We develope a method for single NPC tracking in budding yeast and observe that NPCs exhibit increased mobility in the absence of nuclear basket components. Our data suggest that the distribution of NPCs on the nucleus is governed by the interaction of nuclear basket proteins with the nuclear interior.

### Competing Interest Statement

The authors have declared no competing interest.

*   ### Abbreviations
    
    AID
    :   auxin-inducible degron
    doRITE
    :   dilution of RITE-labelled complexes
    GFP
    :   green fluorescent protein
    NE
    :   nuclear envelope
    NPC
    :   nuclear pore complex
    Nup
    :   nucleoporin
    RFP
    :   red fluorescent protein
    RITE
    :   recombination-induced tag exchange
    SEM
    :   standard error of the mean
    s.d
    :   standard deviation

The nuclear basket regulates the distribution and mobility of nuclear pore complexes in budding yeast

stable-nuclear-basket-proteins-regulate-the-distribution-and-mobility-of-nuclear-pore-complexes-in-budding-yeast


Stable Nuclear Basket Proteins Regulate the Distribution and Mobility of Nuclear Pore Complexes in Budding Yeast
