المفاهيم الأساسية
The author argues that the Hippo pathway regulates cell behavior and density in Capsaspora, indicating an ancient mechanism of cell density control preceding animal multicellularity.
الملخص
The study explores the function of the Hippo pathway in Capsaspora, a close unicellular relative of animals. It reveals that upstream Hippo pathway kinases regulate cell behavior and density within multicellular aggregates. The findings suggest an ancestral role of the Hippo pathway in cytoskeletal regulation and cell density control, independent of proliferation.
The research demonstrates that loss of upstream kinases coHpo and coWts leads to increased nuclear localization of coYki, resulting in contractile cell behavior and higher cell packing density within Capsaspora aggregates. This indicates a conserved regulatory mechanism across species for the Hippo pathway.
Furthermore, the study uncovers a novel role for Hippo signaling in regulating cell density through actomyosin-dependent changes in multicellular architecture. These results provide insights into the evolutionary origins and ancestral functions of developmental pathways like the Hippo cascade.
Overall, the research sheds light on how conserved signaling pathways may have operated in unicellular ancestors, offering valuable insights into the regulation of key cellular processes such as cytoskeletal dynamics and cell density control.
الإحصائيات
Loss of either kinase results in increased nuclear localization of coYki.
Loss of either kinase causes a contractile cell behavior and increased density of cell packing within Capsaspora aggregates.
Increased density is not due to differences in proliferation but actomyosin-dependent changes.
Transgenic expression of hyperactive coYki mutant results in phenotypes similar to mutants lacking coHpo or coWts.
اقتباسات
"The Hippo pathway regulates cytoskeletal dynamics but not proliferation in Capsaspora."
"Loss of upstream kinases leads to increased nuclear localization of coYki."
"Hippo signaling may represent an ancient mechanism for controlling cell density."