
확산 가중 자기공명분광법(dMRS)을 통해 신생아 흰쥐의 소뇌와 시상에서 세포 특이적 발달 궤적을 비침습적으로 추적할 수 있다.


coremsg

It is currently impossible to non-invasively assess cerebellar cell structure during early development. Here we propose a novel approach to non-invasively and longitudinally track cell-specific development using diffusion-weighted magnetic resonance spectroscopy in combination with microstructural modelling. Tracking metabolite diffusion allows us to probe cell-specific developmental trajectories in the cerebellum and thalamus of healthy rat neonates from post-natal day (P) 5 to P30. Additionally, by comparing different analytical and biophysical microstructural models we can follow the differential contribution of cell bodies and neurites during development. The thalamus serves as a control region to assess the sensitivity of our method to microstructural differences between the regions. We found significant differences between cerebellar and thalamic metabolites diffusion properties. For most metabolites, the signal attenuation is stronger in the thalamus, suggesting less restricted diffusion compared to the cerebellum. There is also a trend for lower signal attenuation and lower ADCs with increasing age, suggesting increasing restriction of metabolite diffusion. This is particularly striking for taurine in the thalamus. We use biophysical modelling to interpret these differences. We report a decreased sphere fraction (or an increased neurite fraction) with age for taurine and total creatine in the cerebellum, marking dendritic growth. Surprisingly, we also report a U-shape trend for segment length (the distance between two embranchments in a dendritic tree) in the cerebellum agreeing with age-matching morphometry of openly available 3D-Purkinje reconstructions. Results demonstrate that diffusion-weighted MRS probes early cerebellar neuronal development non-invasively.

### Competing Interest Statement

The authors have declared no competing interest.

Diffusion MRS tracks distinct trajectories of neuronal development in the cerebellum and thalamus of rat neonates

신생아-흰쥐의-소뇌와-시상에서-신경-발달의-다양한-궤적을-추적하는-확산-mrs


신생아 흰쥐의 소뇌와 시상에서 신경 발달의 다양한 궤적을 추적하는 확산 MRS
