Adipokinetic Hormone Signaling Impact on Diaphorina citri Fecundity

Conceptos Básicos

The author argues that adipokinetic hormone signaling plays a crucial role in the fecundity of Diaphorina citri infected by Candidatus Liberibacter asiaticus, impacting lipid metabolism and reproductive behavior.

Resumen

The study explores how CLas infection affects the fecundity of D. citri through AKH/AKHR signaling, miRNA regulation, and JH pathway involvement. CLas-positive psyllids showed increased TAG and glycogen levels, larger lipid droplets, and enhanced fecundity compared to CLas-negative psyllids. Knockdown of DcAKH and DcAKHR resulted in reduced fecundity, altered lipid metabolism, and decreased CLas titers in ovaries. The study highlights a mutualistic interaction between CLas and D. citri ovaries mediated by AKH/AKHR signaling.

Estadísticas

After infection with CLas, the TAG and glycogen levels of CLas-positive psyllids significantly increased.
Knockdown of DcAKH resulted in TAG accumulation and a significant decrease in glycogen.
Knockdown of DcAKHR led to TAG accumulation and a significant decrease in glycogen.
The relative JH titer in the abdomen decreased significantly after dsDcAKH treatment for 48 h.
Knockdown of DcAKHR resulted in a decrease in the expression of JH pathway-related genes.
miR-34 overexpression led to the accumulation of TAG and reduction of glycogen levels.

Citas

"CLas hijacks AKH/AKHR-miR-34-JH signaling to improve D. citri lipid metabolism."
"DcAKHR responds to DcAKH and is involved in the mutualistic relationship between CLas and D. citri."
"miR-34 suppresses DcAKHR expression by binding to its 3’ untranslated region."

Ideas clave extraídas de

Adipokinetic hormone signaling mediates the fecundity of Diaphorina citri infected by ‘Candidatus Liberibacter asiaticus’

by Li,J., Holfo... a las www.biorxiv.org 10-27-2023

https://www.biorxiv.org/content/10.1101/2023.10.24.563719v2

Consultas más profundas

How can understanding vector-pathogen interactions help develop effective control strategies

Understanding vector-pathogen interactions is crucial for developing effective control strategies because it provides insights into how pathogens manipulate their vectors to enhance transmission and spread. By studying the molecular mechanisms underlying these interactions, researchers can identify key pathways that can be targeted for intervention. For example, in the case of D. citri and CLas, this research uncovered how the adipokinetic hormone (AKH) signaling pathway is hijacked by CLas to increase fecundity in D. citri. This knowledge could lead to the development of novel control methods that disrupt this pathway, thereby reducing vector populations or inhibiting pathogen transmission.

What are potential implications for controlling HLB epidemics based on this research

The implications of this research for controlling HLB epidemics are significant. By targeting the AKH/AKHR-miR-34-JH signaling pathway identified in D. citri infected with CLas, new control strategies could be developed to disrupt this mutualistic interaction between the vector and pathogen. For instance, interventions that target components of this pathway could potentially reduce lipid metabolism and fecundity in infected psyllids, leading to decreased transmission rates of CLas and ultimately slowing down the spread of HLB.

How might insights from insect-pathogen interactions be applied to other agricultural pest management practices

Insights from insect-pathogen interactions like those observed in D. citri-CLas system can be applied to other agricultural pest management practices by providing a framework for understanding how pathogens manipulate their vectors for enhanced transmission efficiency. By studying similar interactions in different systems, researchers can uncover common pathways or mechanisms that may be targeted across various vector-borne diseases or pests. This knowledge can inform the development of broad-spectrum control strategies that disrupt key physiological processes involved in pathogen transmission by insect vectors.