
The aggregation of intrinsically disordered protein α-Synuclein is significantly influenced by environmental factors such as molecular crowding and ionic strength, leading to the formation of liquid-liquid phase separated droplets with distinct structural and thermodynamic properties.


coremsg

Intrinsically disordered protein α -Synuclein ( α S) is implicated in Parkinson’s disease due to its aberrant aggregation propensity. In a bid to identify the traits of its aggregation, here we computationally simulate the multi-chain association process of α S in aqueous as well as under diverse environmental perturbations. In particular, the aggregation of α S in aqueous and varied environmental condition led to marked concen-tration differences within protein aggregates, resembling liquid-liquid phase separation (LLPS). Both saline and crowded settings enhanced the LLPS propensity. However, the surface tension of α S droplet responds differently to crowders (entropy-driven) and salt (enthalpy-driven). Conformational analysis reveals that the IDP chains would adopt extended conformations within aggregates and would maintain mutually per-pendicular orientations to minimize inter-chain electrostatic repulsions. The droplet stability is found to stem from a diminished intra-chain interactions in the C-terminal regions of α S, fostering inter-chain residue-residue interactions. Intriguingly, a graph theory analysis identifies small-world-like networks within droplets across environmental conditions, suggesting the prevalence of a consensus interaction patterns among the chains. Together these findings suggest a delicate balance between molecular grammar and environment-dependent nuanced aggregation behaviour of α S.

### Competing Interest Statement

The authors have declared no competing interest.

Modulation of α-Synuclein Aggregation Amid Diverse Environmental Perturbation

molecular-basis-of-α-synuclein-aggregation-and-liquid-liquid-phase-separation-under-environmental-perturbations


Molecular Basis of α-Synuclein Aggregation and Liquid-Liquid Phase Separation under Environmental Perturbations
