מושגי ליבה
The author explores the crucial role of the TOR signaling pathway in regulating vegetative development, aflatoxin biosynthesis, and pathogenicity in Aspergillus flavus through the identification and characterization of key genes involved.
תקציר
The study delves into the intricate mechanisms of the TOR signaling pathway in A. flavus, highlighting its impact on growth, sporulation, sclerotia formation, aflatoxin production, and stress responses. Key findings include the involvement of Fkbp3 in rapamycin resistance and aflatoxin biosynthesis, Sch9 kinase's role in aflatoxin synthesis and stress response regulation, TipA's influence on sclerotia development and cell wall stress response, as well as SitA and Ppg1's critical functions in growth, conidiation, sclerotial formation, aflatoxin production, pathogenicity, lipid droplet biogenesis regulation, and cell wall integrity maintenance.
סטטיסטיקה
Rapamycin at 100 ng/mL significantly inhibited mycelial growth and conidia formation.
Deletion of Fkbp3 led to increased resistance to rapamycin.
The Δsch9 strain exhibited reduced aflatoxin production compared to the wild-type strain.
The ΔtipA strain showed decreased sensitivity to rapamycin but was unable to produce sclerotia.
Deletion mutants of sitA and ppg1 displayed slower growth on PDA medium.
The Δnem1 and Δspo7 mutants exhibited reduced vegetative growth and conidiation compared to the wild-type strain.
ציטוטים
"The TOR signaling pathway is involved in multiple cellular processes in A. flavus."
"Fkbp3 plays a crucial role in sclerotia formation and aflatoxin biosynthesis."
"Sch9 regulates aflatoxin biosynthesis by modulating various stresses."