
filopodia 내에는 수백 개의 myosin 10 분자가 밀집되어 있으며, 이는 actin 위에 교통 체증을 일으킬 수 있다.


coremsg

Myosin 10 (Myo10) is a motor protein well known for its role in filopodia formation. Although Myo10-driven filopodial dynamics have been characterized, there is no information about the absolute number of Myo10 molecules during the filopodial lifecycle. To better understand molecular stoichiometries and packing restraints in filopodia, we measured Myo10 abundance in these structures. Here we combined SDS-PAGE densitometry with epifluorescence microscopy to quantitate HaloTag-labeled Myo10 in U2OS cells. About 6% of total intracellular Myo10 localizes to filopodia, where it is enriched at opposite ends of the cell. Hundreds of Myo10 are found in a typical filopodium, and their distribution across filopodia is log-normal. Some filopodial tips even contain more Myo10 than accessible binding sites on the actin filament bundle. Live-cell movies reveal a dense cluster of over a hundred Myo10 molecules that initiates filopodial elongation. Hundreds of Myo10 molecules continue to accumulate during filopodial growth, but that accumulation ceases when filopodia begin to retract. Rates of filopodial elongation, second-phase elongation, and retraction are inversely related to Myo10 quantities. Our estimates of Myo10 molecules in filopodia provide insight into the physics of packing Myo10, its cargo, and other filopodia-associated proteins in narrow membrane compartments. Our protocol provides a framework for future work analyzing Myo10 abundance and distribution upon perturbation.

### Competing Interest Statement

RSR is a consultant for Cyntegron Therapeutics.

Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actin

세포-내-수백-개의-myosin-10이-filopodia에-밀집되어-있어-actin-위에-교통-체증을-일으킬-수-있다


세포 내 수백 개의 myosin 10이 filopodia에 밀집되어 있어 actin 위에 교통 체증을 일으킬 수 있다
