
DC-iDEP 기술을 활용하여 포도당 자극에 따른 인슐린 분비 소포체의 생물리학적 특성 변화를 규명하였다.


coremsg

Organelle heterogeneity and inter-organelle associations within a single cell contribute to the limited sensitivity of current organelle separation techniques, thus hindering organelle subpopulation characterization. Here we use direct current insulator-based dielectrophoresis (DC-iDEP) as an unbiased separation method and demonstrate its capability by identifying distinct distribution patterns of insulin vesicles from pancreatic β-cells. A multiple voltage DC-iDEP strategy with increased range and sensitivity has been applied, and a differentiation factor (ratio of electrokinetic to dielectrophoretic mobility) has been used to characterize features of insulin vesicle distribution patterns. We observed a significant difference in the distribution pattern of insulin vesicles isolated from glucose-stimulated cells relative to unstimulated cells, in accordance with functional maturation of vesicles upon glucose stimulation, and interpret this to be indicative of high-resolution separation of vesicle subpopulation. DC-iDEP provides a path for future characterization of subtle biochemical differences of organelle subpopulations within any biological system.

### Competing Interest Statement

MAH declares a conflict of interest with CBio where he serves as the CSO and with Hayes Diagnostics, Inc. where he serves as COB, CEO & CSO. All other authors have no conflict of interest to declare.

iDEP-assisted isolation of insulin secretory vesicles

인슐린-분비-소포체의-dc-idep-기반-분리를-통한-특성-규명


인슐린 분비 소포체의 DC-iDEP 기반 분리를 통한 특성 규명
