toplogo
Sign In

Sibling Chimerism in Marmoset Microglia


Core Concepts
Sibling-derived chimerism in marmoset microglia arises from hematopoietic cells, providing insights into brain biology and gene effects.
Abstract
Chimerism common in marmosets due to fraternal twin birth patterns. Sibling-derived chimerism found in microglia and macrophages but not other brain cell types. Brain regions show varying levels of sibling contribution to microglial populations. Genetic differences have a smaller impact on gene expression than contextual factors. Chimerism offers unique opportunities for studying cell migration, recruitment, and gene effects.
Stats
"The presence of Y-chromosome DNA sequences in other organs of female marmosets has long suggested that chimerism might also affect these organs." "Microglia exhibited abundant chimerism – across the 11 marmosets, the total fraction of cells with the sibling’s genome ranged from 20% to 52%."
Quotes
"Chimerism provides the unusual opportunity to compare cells with different genomes in a shared in vivo biological context." "Natural chimerism may enable many straightforward ways to pursue studies distinguishing cell-autonomous from non-cell autonomous genetic effects."

Key Insights Distilled From

by del Rosario,... at www.biorxiv.org 10-17-2023

https://www.biorxiv.org/content/10.1101/2023.10.16.562516v1
Sibling chimerism among microglia in marmosets

Deeper Inquiries

How does sibling-derived chimerism impact our understanding of brain disorders

Sibling-derived chimerism in the brain provides a unique opportunity to deepen our understanding of various brain disorders. By analyzing microglia and macrophages with different genomes within the same brain, researchers can investigate how genetic variations impact cellular function and behavior. This insight is crucial for unraveling the complex mechanisms underlying conditions such as Alzheimer's disease, Parkinson's disease, autism spectrum disorder, and schizophrenia. Understanding how sibling-derived cells interact within the brain can shed light on the role of genetics in these disorders and potentially lead to new therapeutic approaches.

What are the implications of genetic differences versus contextual factors on gene expression

The comparison between genetic differences and contextual factors on gene expression offers valuable insights into cellular biology. By studying microglia with host versus sibling genomes in shared environments, researchers can distinguish between cell-autonomous effects (genetic differences) and non-cell autonomous effects (contextual factors). This distinction is essential for determining how much of gene expression regulation is driven by intrinsic genetic factors versus external environmental cues. Understanding this balance can provide critical information on how genes interact with their surroundings to influence cellular function.

How can natural chimerism be leveraged for future research beyond marmoset biology

Natural chimerism presents exciting opportunities for future research beyond marmoset biology. Leveraging chimeric tissues allows scientists to study the impact of mutations, gene expression patterns, and cell interactions in a unique biological context where cells have different genomes but share a common environment. This approach could be instrumental in investigating developmental processes, immune responses, tissue regeneration, and disease progression across various organisms. Additionally, natural chimerism provides a powerful tool for exploring the interplay between genetics and environment in shaping biological outcomes at a cellular level.
0