Core Concepts
The author explores the dual functionality of zebrafish kidney as a hematopoietic hub and secondary lymphoid organ, highlighting its unique immune system features.
The main thesis is that zebrafish kidney serves as a crucial site for both hematopoiesis and adaptive antiviral immunity.
Abstract
The content delves into the unique role of zebrafish kidney in generating blood cell lineages and mounting immune responses. Through single-cell transcriptome profiling, 59 hematopoietic stem/progenitor cell (HSPC) and immune cell types were identified. These populations displayed differential responses to viral infection, indicating diverse functional roles in antiviral immunity. HSPCs showed extensive reactivity to viral infection, inducing trained immunity. The antigen-stimulated adaptive immunity was fully generated in the kidney, suggesting its function as a secondary lymphoid organ. The study provides insights into the multifaceted biology of fish immune systems.
Stats
The control group exhibited mean reads/median genes per cell of 34,501/1,090.
The infected group showed 29,706/1,383.
The vaccinated+infected group displayed 37,611/1,379.
Total number of detected genes in leukocyte samples from control, infected, and vaccinated+infected groups were 26,345, 26,865, and 25,342 respectively.
Quotes
"Our findings illustrated the unique features of fish immune system."
"HSPCs were found to have extensive reactivities to viral infection."