Core Concepts
Efficient endogenous tagging using split mNeonGreen enables live imaging studies of cellular processes in human iPSCs.
Stats
Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in HEK293 cells.
Large-scale endogenous tagging in HEK293 cells was recently achieved by the OpenCell project, with 1,310 proteins tagged to date.
Quotes
"The two fragments have been engineered to form a functional fluorescent protein when co-expressed."
"This work represents the first step towards a genome-wide endogenous tag library in human stem cells."