The study explores how centrosome age influences spindle symmetry in human epithelial and fibroblastic cells. Centrosomes of different ages lead to subtle spindle asymmetries, affecting daughter cell sizes. Pericentrin, Cdk5Rap2, and γ-tubulin accumulate preferentially on old centrosomes, impacting microtubule nucleation. The presence of daughter centrioles dampens the asymmetry induced by grandmother centrioles. Depletion of pericentrin or TPX2 restores spindle symmetry, highlighting their role in breaking spindle symmetry based on centrosome age. Additionally, Plk1 binding to cenexin drives spindle size and polar chromosome asymmetries.
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by Thomas,A., M... at www.biorxiv.org 09-15-2023
https://www.biorxiv.org/content/10.1101/2023.09.15.557935v1Deeper Inquiries