Core Concepts
Distinct and tissue-specific responses of regulatory T cells to antigen challenges are highlighted through TCR repertoire analysis.
Abstract
The suppressive function of regulatory T cells (Treg) relies on antigen receptor signaling triggered by various antigens. A mouse model with a fixed TCRβ chain was used to analyze conventional and regulatory effector TCRα repertoires in response to different lung and skin challenges. The study revealed challenge-specific clonal expansions within tissues, draining lymph nodes, and across animals. Some clusters were shared across cancer challenges, indicating a response to common tumor-associated antigens. The distinct origin of eCD4 and eTreg subsets was observed for most challenges, but overlap was noted at certain tumor sites. Additionally, the study demonstrated that resident eTreg populations have distinct antigenic specificities based on their tissue location.
Stats
524 TCRα cDNA libraries sequenced
13,605 ± 14,948 UMI-labeled TCRα cDNA molecules per eTreg sample
3,392 ± 3,076 TCRα CDR3 clonotypes per eTreg sample
37,412 ± 33,129 UMI-labeled TCRα cDNA molecules per eCD4 sample
5,518 ± 4,601 TCRα CDR3 clonotypes per eCD4 sample
Quotes
"Tissue-specific responses of regulatory T cells associated with distinct clonal expansions."
"Distinct antigenic specificities characterize resident tissue-specific regulatory T cell populations."