The study investigates a previously unrecognized subset of cerebellar nuclei (CN) neurons that do not originate from the well-established germinal zones of the cerebellar primordium. Key findings:
A subset of CN neurons expressing SNCA, OTX2, MEIS2, and p75NTR are located in the rostroventral region of the nuclear transitory zone (NTZ) in the cerebellar primordium, distinct from the rhombic lip-derived CN neurons.
The SNCA+/OTX2+/MEIS2+/p75NTR+ CN neurons do not originate from the cerebellar ventricular zone or rhombic lip, as confirmed using Otx2-GFP and Atoh1-/- mice.
Embryonic DiI labeling experiments suggest the mesencephalon as a potential extrinsic germinal zone for this novel subset of CN neurons.
The SNCA+/OTX2+/MEIS2+/p75NTR+ CN neurons are present in the absence of Atoh1, indicating their development is independent of the rhombic lip-derived CN neurons.
These results reveal the existence of a previously unidentified germinal zone, potentially the mesencephalon, that gives rise to a distinct subset of CN neurons during early cerebellar development.
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by Rahimi-Balae... at www.biorxiv.org 11-01-2017
https://www.biorxiv.org/content/10.1101/212571v2Deeper Inquiries