Core Concepts
A single exposure to drugs of abuse or anesthetic agents like ketamine/xylazine induces widespread and long-lasting changes in the brain-wide input patterns to ventral tegmental area dopamine neurons, which may contribute to the development of addiction-related behaviors.
Abstract
The study used a rabies virus-based mapping strategy to quantify the brain-wide inputs to ventral tegmental area (VTA) dopamine, GABA, and glutamate neurons after a single exposure to various drugs of abuse (cocaine, amphetamine, methamphetamine, morphine, nicotine) or the anesthetic agent ketamine/xylazine (K/X).
Key findings:
All drugs of abuse induced similar changes in input patterns to VTA dopamine neurons, particularly from regions involved in stress and reward processing.
K/X anesthesia also induced long-lasting changes in input patterns, which overlapped with but were distinct from the changes induced by drugs of abuse.
The input changes induced by K/X anesthesia were more widespread, targeting both dopamine and non-dopamine VTA neurons.
The extent of drug- or K/X-induced input changes was correlated with the basal expression levels of genes related to synaptic transmission, plasticity, and ion channels, especially calcium channels.
These results indicate that even a single drug exposure can trigger widespread and persistent changes in brain connectivity, and highlight potential concerns about the use of K/X anesthesia in rodent studies of motivated behaviors.
Stats
Cocaine was administered at 15 mg/kg.
Morphine was administered at 10 mg/kg.
Methamphetamine was administered at 2 mg/kg.
Amphetamine was administered at 10 mg/kg.
Nicotine was administered at 0.5 mg/kg.
Fluoxetine was administered at 10 mg/kg.
Quotes
"Exposure to drugs of abuse causes long-lasting changes in connectivity to ventral tegmental area dopamine cells that contribute to drug-induced behavioral adaptations."
"Ketamine/xylazine anesthesia induces a similar but different set of long-lasting input changes onto midbrain dopamine cells, indicating that caution should be taken when using ketamine/xylazine-based anesthesia in rodents when assessing motivated behaviors."