The content discusses the protective effects of eugenol (EUG), a natural compound, on type 1 diabetes mellitus (T1DM). The key highlights are:
In vivo experiments using a streptozotocin (STZ)-induced T1DM mouse model showed that EUG intervention could effectively ameliorate the symptoms associated with T1DM, including polydipsia, polyphagia, polyuria, and weight loss. EUG also improved islet function and insulin secretion in T1DM mice.
EUG treatment reduced apoptosis and DNA damage in pancreatic β cells of T1DM mice, as evidenced by decreased expression of γH2AX, BAX, and Cleaved Caspase-3, as well as increased BCL2 expression.
Mechanistically, EUG activated the nuclear factor E2-related factor 2 (NRF2) signaling pathway, leading to increased expression of downstream antioxidant proteins NQO-1 and HO-1. This helped alleviate oxidative stress in T1DM mice, as shown by reduced MDA levels and increased SOD, CAT, and GSH-Px levels.
In vitro experiments using STZ-induced MIN6 pancreatic β cells confirmed that EUG could protect against STZ-induced cell damage by activating the NRF2 pathway and reducing oxidative stress and apoptosis. The protective effects of EUG were reversed by the NRF2 inhibitor ML385.
Overall, the study suggests that EUG has the potential to be a therapeutic candidate for T1DM by mitigating pancreatic β cell damage through the NRF2-mediated oxidative stress pathway.
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biorxiv.org
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by Jiang,Y., He... at www.biorxiv.org 03-11-2024
https://www.biorxiv.org/content/10.1101/2024.03.06.583719v1Deeper Inquiries