
Direct current insulator-based dielectrophoresis (DC-iDEP) can separate distinct subpopulations of insulin secretory vesicles from pancreatic beta cells, with glucose stimulation altering the biophysical properties and distribution patterns of these vesicle subpopulations.


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Organelle heterogeneity and inter-organelle associations within a single cell contribute to the limited sensitivity of current organelle separation techniques, thus hindering organelle subpopulation characterization. Here we use direct current insulator-based dielectrophoresis (DC-iDEP) as an unbiased separation method and demonstrate its capability by identifying distinct distribution patterns of insulin vesicles from pancreatic β-cells. A multiple voltage DC-iDEP strategy with increased range and sensitivity has been applied, and a differentiation factor (ratio of electrokinetic to dielectrophoretic mobility) has been used to characterize features of insulin vesicle distribution patterns. We observed a significant difference in the distribution pattern of insulin vesicles isolated from glucose-stimulated cells relative to unstimulated cells, in accordance with functional maturation of vesicles upon glucose stimulation, and interpret this to be indicative of high-resolution separation of vesicle subpopulation. DC-iDEP provides a path for future characterization of subtle biochemical differences of organelle subpopulations within any biological system.

### Competing Interest Statement

MAH declares a conflict of interest with CBio where he serves as the CSO and with Hayes Diagnostics, Inc. where he serves as COB, CEO & CSO. All other authors have no conflict of interest to declare.

iDEP-assisted isolation of insulin secretory vesicles

dielectrophoretic-separation-reveals-distinct-subpopulations-of-insulin-secretory-vesicles-in-pancreatic-beta-cells


Dielectrophoretic Separation Reveals Distinct Subpopulations of Insulin Secretory Vesicles in Pancreatic Beta Cells
