核心概念
CK-666 and CK-869 have differential effects on Arp2/3 iso-complexes, impacting actin nucleation and branching.
要約
Abstract:
CK-666 and CK-869 are inhibitors used to study actin nucleation by the Arp2/3 complex.
Recombinant complexes were used to assess the impact of these inhibitors on different iso-complexes.
Introduction:
The Arp2/3 complex initiates actin filament formation in cells.
Data Extraction:
"CK-666 binds between Arp2 and Arp3."
Quotations:
"Our analyses have important implications for the interpretation of results using CK-666 and CK-869."
Results and Discussion:
CK-869 Impact on Vaccinia-induced Actin Polymerization:
CK-869 inhibits vaccinia-induced actin assembly, unlike CK-666.
CK-666 vs. CK-869 on ArpC1A-containing Complexes:
Both drugs inhibit actin polymerization mediated by ArpC1A complexes.
Macrophage Phagocytosis and Motility:
CK-669 has no effect on macrophage motility but impacts phagocytosis.
Impact on F-Actin Binding and VCA Interaction:
Neither drug affects the interaction between Arp2/3 complex and actin filaments or VCA binding.
SPIN90 Activation of Iso-complexes:
Both drugs inhibit SPIN90-mediated activation of all iso-complexes.
Inhibition of Arp3B-containing Complexes:
CK-669 cannot inhibit Arp3B-containing complexes, unlike its effect on other iso-complexes.
Conclusion:
The study highlights the distinct effects of CK-666 and CK-869 on different iso-complexes, providing insights into their mechanisms of action.
統計
"CK-666 binds between Arp2 and Arp3."
引用
"Our analyses have important implications for the interpretation of results using CK-666 and CK-869."