The authors conducted a genome-wide association study (GWAS) using the Drosophila Genetic Reference Panel (DGRP) to identify the genetic basis of diapause in Drosophila melanogaster. They quantified diapause by assessing the ability of flies to undergo 35 days of diapause, recover, and produce viable progeny, which is a more stringent test of diapause success compared to previous studies.
The GWAS revealed 546 genetic variants associated with post-diapause fecundity, encompassing 291 candidate diapause-associated genes. Many of these genes were previously implicated in diapause, while others were newly identified. Gene network analysis showed that the diapause-associated genes were primarily linked to neuronal and reproductive system development.
The authors further complemented the genetic analysis by identifying specific neurons required for successful diapause. They found that blocking neuronal transmission in olfactory receptor neurons and temperature-sensing neurons impaired recovery from diapause. Removing the antenna, which houses these sensory neurons, reduced diapause lifespan and post-diapause fecundity, suggesting the olfactory system plays a critical role in diapause.
RNAi knockdown experiments identified two neuronal genes, Dip-γ and Scribbler, as required during recovery for post-diapause fecundity. These findings provide insights into the molecular, cellular, and genetic basis of adult reproductive diapause in Drosophila.
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by Easwaran,S.,... 場所 www.biorxiv.org 03-12-2024
https://www.biorxiv.org/content/10.1101/2024.03.10.584341v1深掘り質問