A Novel Method for Clearing and Labeling FFPE Brain Tissues

核心概念

The author introduces MOCAT as a method to efficiently clear and label FFPE brain tissues, enabling comprehensive 3D analyses. By optimizing antigen retrieval with heat-induced methods, MOCAT allows for deep immunolabeling and volumetric quantification of neuronal circuitry in archived specimens.

要約

Tissue clearing techniques have transformed brain-wide imaging, but applying them to FFPE blocks remains challenging. MOCAT offers a solution by efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. This method enables multi-round immunolabeling, revealing neuron circuitry in whole FFPE mouse brains and supporting expansion microscopy. The study showcases the broad applications of MOCAT for researching archived FFPE specimens in neuroscientific and neuropathological analyses. The pipeline involves depaffination, rehydration, epoxy processing, optimized antigen retrieval, electrophoretic immunolabeling, refractive index matching, volumetric imaging, 3D visualization, photobleaching, Raman microscopy, and more.

Key points:

• Introduction of MOCAT for efficient clearing and labeling of FFPE brain tissues.
• Optimization of antigen retrieval with heat-induced methods.
• Multi-round immunolabeling reveals neuron circuitry in whole FFPE mouse brains.
• Supports expansion microscopy and long-term storage samples.
• Potential applications in neuroscientific research and pathology.

統計

"MOCAT represents a feasible approach for researching archived FFPE specimens."
"MOCAT enables multi-round staining on intact FFPE mouse brains."
"1% Tween 20 condition presented significantly lower lipid content compared to the control group."
"MOCAT+ allows multi-round immunolabeling on human brain tissues."

引用

"By optimizing antigen retrieval with heat-induced methods, MOCAT allows for deep immunolabeling and volumetric quantification of neuronal circuitry in archived specimens."
"MOCAT offers a solution by efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents."

抽出されたキーインサイト

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

by Lin,Y.-H., W... 場所 www.biorxiv.org 12-02-2022

https://www.biorxiv.org/content/10.1101/2022.11.28.518189v4

深掘り質問

How can MOCAT be adapted for use on tissues other than the brain?

MOCAT can be adapted for use on tissues other than the brain by making some modifications to suit the specific characteristics of different tissue types. Here are some ways in which MOCAT can be adjusted for broader tissue applications:

Optimized Antigen Retrieval Solutions: Different tissues may require varying antigen retrieval solutions based on their composition and structure. By testing and optimizing antigen retrieval buffers, MOCAT can be tailored to effectively retrieve antigens from various tissue types.

Staining Strategies: The staining strategies used in MOCAT, such as passive and active immunolabeling, may need adjustments depending on the tissue's thickness and properties. Fine-tuning these strategies will ensure efficient penetration of antibodies into different tissues.

Epoxy or Hydrogel Pre-Treatments: For certain tissues that might require additional support during staining procedures, pre-treatments with epoxy or hydrogels could enhance structural integrity and facilitate better antibody penetration.

Refractive Index Matching: RI matching solutions used in MOCAT may need customization based on the optical properties of different tissues to achieve optimal transparency for imaging purposes.

Imaging Techniques: Depending on the nature of the tissue being studied, appropriate imaging techniques should be selected to capture detailed 3D information accurately.

By adapting these aspects of the MOCAT pipeline according to specific tissue requirements, researchers can extend its application beyond neuroscience research to a wide range of biological samples.

What are the implications of using MOCAT for long-term fixed specimens?

Using MOCAT for long-term fixed specimens has several significant implications:

Preservation of Archival Samples: Long-term fixed specimens stored in FFPE blocks represent valuable archival resources that often contain crucial information about diseases or conditions over time. Applying MOCAT allows researchers to access this preserved data without compromising sample integrity.

Retrospective Studies: With MOCAT, archived FFPE specimens can be retrospectively analyzed using advanced imaging techniques like multiplexed immunolabeling and 3D visualization even after years of storage. This enables researchers to revisit past studies or investigate new research questions without needing fresh samples.

Comparative Analyses : By applying consistent protocols like Mocat across both freshly processed samples and long-term fixed specimens, researchers can conduct comparative analyses between different time points or experimental conditions more effectively while ensuring data consistency.

4 .Longitudinal Research: Long-term fixed specimens allow longitudinal studies tracking disease progression or treatment outcomes over extended periods.Mocat facilitates comprehensive analysis at multiple time points within a single specimen,revealing changes over time
Overall,Mocat enhances accessibility,reproducibility,and robustness when working with long term-fixed samples,enabling deeper insights into disease mechanisms,treatment responses,and pathological changes.

How might integration with spatial omics techniques enhance utility beyond neuroscience research?

Integrating MOCAt with spatial omics techniques offers several advantages that extend its utility beyond neuroscience research:
1 .Comprehensive Molecular Profiling: Spatial omics techniques enable simultaneous profilingof multiple molecular features within intact biological structures.By combining MOCA twith spatial transcriptomicsor proteomics,researcherscan obtain detailed informationon gene expression patternsand protein distributionsacross entiretissues.This holistic approach providesa more comprehensive understandingof cellular interactionsand functional relationshipswithin complexbiological systemsbeyond what traditional methods offer
2 .Cellular Heterogeneity Analysis: Spatial omicstechniques coupledwith MOCA Tallowfor high-resolutionmappingof celltypesand subpopulationsin contextwithin intacttissues.Thisenablesdetailed characterizationof cellularheterogeneity,spatial organization,and intercellularcommunicationacrossdifferent regions.Theseinsightsare criticalfor unravelingcomplexbiological processesin health,disease,and developmentbeyondneuroscienceapplications
3 .Therapeutic Target Identification: IntegrationofMOCA Twithspatialomicstechniquescan aidin identifyingnovel therapeutic targetsby correlatingmolecular signatureswith morphologicalfeaturesandspecificcellularcontexts.Withthisapproach,researcherscan pinpointtargetsof interestmore preciselyandinformedecision-makingregardingpotential treatmentsor interventionsfor variousconditionsoutside neuroscienceresearchareas
4 .Clinical Applications: The combinationofMO CA Tandspatialomicstechniqueshas promisingclinical applications,suchas diagnostictesting,personalizedmedicine,and biomarkerdiscovery.Incorporatingtheseadvancedmethodologiesinto clinicalresearchallowsfor enhancedcharacterizationoftissuearchitecture,functionalandmolecularprofiles,influencingdiagnosis,treatmentstrategies,andpatientoutcomesbeyondthe realmofneurological disorders