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Presynaptic Rac1 Regulates Spatial Working Memory in the Hippocampus


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Presynaptic Rac1 inhibition selectively impairs spatial working memory, while postsynaptic Rac1 inhibition affects remote memory, suggesting a site-specific role of Rac1 in hippocampal-dependent cognitive processes.
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The study explores the behavioral effects and regulatory mechanisms of the small Rho-family GTPase Rac1 at presynaptic terminals in the hippocampus. Key findings:

  1. Presynaptic Rac1 inhibition selectively impairs spatial working memory in mice, without affecting other types of memory like spatial reference memory and fear memory. In contrast, postsynaptic Rac1 inhibition impairs remote fear memory.

  2. Electron microscopy analysis reveals that presynaptic Rac1 inhibition affects synaptic structures, including an increase in dendritic spine size and changes in synaptic vesicle morphology and distribution.

  3. Proteomic mapping of presynaptic Rac1 interactions identifies key kinases like Mink1 and Group I PAKs that may phosphorylate synaptic proteins like Syntaxin-1b and Synaptotagmin-1. These phosphorylation events could regulate synaptic vesicle dynamics and contribute to the observed effects on spatial working memory.

The findings suggest a compartmentalized role of Rac1 in hippocampal-dependent cognitive processes, with presynaptic Rac1 selectively regulating spatial working memory through phosphoregulation of synaptic proteins and modulation of synaptic vesicle organization and dynamics.

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Presynaptic Rac1 inhibition leads to a decrease in the number of arm entries before the first error and a decrease in the latency to the first error in the radial-arm maze test. Presynaptic Rac1 inhibition results in a decrease in the percentage of spontaneous alternations in the Y-maze test. Presynaptic Rac1 inhibition leads to an increase in the diameter of synaptic vesicles and a greater distance of vesicles from the active zone in the hippocampal CA1 region. Presynaptic Rac1 inhibition results in an increase in the area of dendritic spines in the hippocampal CA1 region.
Citaten
"Presynaptic Rac1 inhibition selectively impairs spatial working memory, while postsynaptic Rac1 inhibition affects remote memory, suggesting a site-specific role of Rac1 in hippocampal-dependent cognitive processes." "Our findings suggest that the presynaptic functions of Rac1 are intricately mediated through the phosphorylation of synaptic vesicle proteins. This phosphoregulation, coupled with actin cytoskeleton remodeling, may play a pivotal role in modulating the synaptic vesicle cycle and plasticity, underscoring a sophisticated mechanism by which Rac1 may influence synaptic dynamics and, consequently, cognitive processes."

Belangrijkste Inzichten Gedestilleerd Uit

by Kim,J., Bust... om www.biorxiv.org 03-19-2024

https://www.biorxiv.org/content/10.1101/2024.03.18.585488v1
Presynaptic Rac1 in the hippocampus selectively regulates working memory

Diepere vragen

How do the site-specific effects of Rac1 on working memory and remote memory relate to the distinct neural circuits and signaling pathways involved in these different memory types?

The site-specific effects of Rac1 on working memory and remote memory can be attributed to the involvement of distinct neural circuits and signaling pathways in these different memory types. Working memory, which is essential for temporary storage and manipulation of information, relies heavily on the prefrontal cortex and its connections with the hippocampus. The hippocampus plays a crucial role in spatial working memory, as seen in tasks like the radial-arm maze and Y-maze. In contrast, remote memory, which involves the retrieval of memories formed in the past, is more reliant on the neocortex and its connections with subcortical structures. Presynaptic Rac1 inhibition specifically affecting spatial working memory suggests that Rac1 signaling at presynaptic terminals in the hippocampus plays a critical role in the processes underlying spatial navigation and memory retention over short periods. The selective impairment of working memory without affecting other memory forms indicates a compartmentalized function of Rac1 in cognitive processes. On the other hand, postsynaptic Rac1 inhibition impacting remote memory but not working memory suggests that Rac1 signaling at postsynaptic sites may be more involved in the consolidation and retrieval of long-term memories. The distinct neural circuits involved in working memory and remote memory likely interact with different downstream signaling pathways influenced by Rac1 activity. The differential effects of Rac1 inhibition on these memory types highlight the complexity of memory processes and the specific roles of Rac1 in modulating synaptic plasticity and cognitive functions in a site-specific manner.

How do the potential compensatory mechanisms or alternative pathways allow for the preservation of long-term memory despite the impairment in spatial working memory following presynaptic Rac1 inhibition?

The preservation of long-term memory despite the impairment in spatial working memory following presynaptic Rac1 inhibition may involve compensatory mechanisms and alternative pathways that enable the brain to adapt and maintain cognitive functions. One possible compensatory mechanism could be the recruitment of alternative neural circuits or synaptic pathways to compensate for the deficits in spatial working memory caused by presynaptic Rac1 inhibition. The brain has the remarkable ability to reorganize and redistribute cognitive functions to different regions or networks in response to disruptions in specific pathways. Additionally, other molecular signaling pathways and neurotransmitter systems may come into play to support long-term memory formation and retrieval in the absence of optimal spatial working memory function. For example, the activation of alternative synaptic plasticity mechanisms, such as long-term potentiation (LTP) or long-term depression (LTD), in different brain regions could contribute to the maintenance of long-term memory despite the impairment in working memory. Furthermore, the complex interplay between various neurotransmitters, neuromodulators, and neurotrophic factors may also play a role in sustaining long-term memory processes. These factors can modulate synaptic strength, neuronal excitability, and synaptic connectivity to compensate for deficits in specific cognitive functions. Overall, the brain's plasticity and ability to adapt to changes in neural activity patterns allow for the preservation of long-term memory even in the presence of impairments in spatial working memory following presynaptic Rac1 inhibition.

Could the phosphoregulation of synaptic proteins like Syntaxin-1b and Synaptotagmin-1 by Rac1-associated kinases be targeted as a therapeutic strategy to modulate synaptic function and cognitive processes in neurological disorders?

The phosphoregulation of synaptic proteins like Syntaxin-1b and Synaptotagmin-1 by Rac1-associated kinases presents a promising avenue for therapeutic interventions to modulate synaptic function and cognitive processes in neurological disorders. Targeting the phosphorylation of key synaptic proteins involved in neurotransmitter release and synaptic vesicle dynamics could offer a novel approach to modulate synaptic plasticity and cognitive functions in conditions characterized by synaptic dysfunction. By manipulating the activity of Rac1-associated kinases that phosphorylate proteins like Syntaxin-1b and Synaptotagmin-1, it may be possible to fine-tune synaptic transmission, vesicle release, and neurotransmitter release, which are essential for proper neuronal communication and cognitive processes. Dysregulation of these processes is implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and schizophrenia. Therapeutic strategies aimed at targeting the phosphorylation of synaptic proteins by Rac1-associated kinases could involve the development of small molecule inhibitors or activators that modulate the activity of these kinases. By selectively regulating the phosphorylation status of key synaptic proteins, it may be possible to restore normal synaptic function, enhance synaptic plasticity, and improve cognitive processes in individuals with neurological disorders. Overall, the phosphoregulation of synaptic proteins by Rac1-associated kinases represents a promising therapeutic target for the development of novel treatments for neurological disorders that involve synaptic dysfunction and cognitive impairment.
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