Główne pojęcia
Neuropeptide Bursicon and its receptor CcBurs-R play a critical role in regulating the transition from summer-form to winter-form in the pear psylla Cacopsylla chinensis by affecting cuticle content and thickness.
Streszczenie
This study investigated the novel function of neuropeptide Bursicon and its receptor in mediating the seasonal polyphenism of the pear psylla Cacopsylla chinensis.
The authors first identified two Bursicon subunits, CcBurs-α and CcBurs-β, and found that their expression was upregulated by low temperature (10°C) and the temperature receptor CcTRPM. Knockdown of CcBurs-α, CcBurs-β, or their receptor CcBurs-R significantly reduced cuticle pigment content, chitin content, and cuticle thickness, leading to a decreased transition from summer-form to winter-form in C. chinensis.
Further experiments revealed that the Bursicon heterodimer (CcBurs-α+β) could activate CcBurs-R, which acted as the upstream regulator of the chitin biosynthesis pathway. Additionally, the microRNA miR-6012 was found to directly target CcBurs-R and inhibit its expression, contributing to the regulation of Bursicon signaling in the seasonal polyphenism of C. chinensis.
These findings provide novel insights into the neuroendocrine mechanisms underlying insect seasonal polyphenism, highlighting the critical role of Bursicon signaling in mediating the transition from summer-form to winter-form in the pear psylla.
Statystyki
Low temperature (10°C) significantly increased the mRNA expression of CcBurs-α, CcBurs-β, and CcBurs-R compared to 25°C.
Knockdown of CcBurs-α, CcBurs-β, or CcBurs-R reduced the total cuticle pigment absorbance by 78.8%, 77.6%, and 83.5%, respectively, compared to the control.
Knockdown of CcBurs-α, CcBurs-β, or CcBurs-R decreased the cuticle chitin content by 67%, 68%, and 69%, respectively, compared to the control.
Knockdown of CcBurs-α, CcBurs-β, or CcBurs-R reduced the cuticle thickness by 57.5%, 54.9%, and 60.5%, respectively, compared to the control.
Knockdown of CcBurs-α, CcBurs-β, or CcBurs-R decreased the transition percent from summer-form to winter-form by 69.6%, 69.0%, and 68.1%, respectively, compared to the control.
Cytaty
"Bursicon, also known as the tanning hormone, was initially discovered in the 1960s through neck-ligated assays. It serves a highly conserved function in insects by inducing the clerotization and melanization of the new cuticle in larvae and facilitating wing expansion in adults."
"Increasing studies have shown that miRNAs are important in insect polyphenism, such as miR-31, miR-9, and miR-252, as well as hormone signaling, for examples, miR-133 in dopamine synthesis."
"These findings reveal the novel function of neuroendocrine regulatory mechanism underlying seasonal polyphenism and provide critical insights into insect Bursicon and its receptor."