The content describes the development and validation of a novel electrolytic lesioning technique that can be used in conjunction with chronically implanted multielectrode arrays. The key highlights are:
The authors created a custom current source circuit that allows for controlled, repeatable electrolytic lesions through the same multielectrode array used for neuronal activity recordings. This enables stable electrophysiological recordings before and after the lesion.
Ex vivo testing in sheep and pig brains, as well as in vivo testing in anesthetized pigs, demonstrated that the size and extent of the lesion can be controlled by adjusting the amplitude and duration of the current passed through the electrodes. Smaller, more focal lesions were achieved by reducing the current intensity and duration.
The technique was validated in vivo in sedated and awake-behaving rhesus macaques, showing that it can be safely used in primates without compromising the ability to record neuronal activity.
Analysis of the recorded neuronal activity before and after the lesions revealed changes in the proportions of recorded neurons, likely reflecting neuron loss or silencing within the lesioned area.
The authors show that this electrolytic lesioning technique can be used with different types of multielectrode probes, demonstrating its flexibility and cross-compatibility.
This novel lesioning method provides a powerful tool for establishing causal links between neuronal circuit activity and behavior, while also enabling the study of local reorganization and recovery after neuron loss.
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by Bray,I. E., ... o www.biorxiv.org 11-13-2022
https://www.biorxiv.org/content/10.1101/2022.11.10.516056v2Głębsze pytania