
나노포어 직접 RNA 분석을 위한 수정되지 않은 인간 전사체 데이터셋을 제공하여 RNA 수식화 분석을 지원한다.


coremsg

Nanopore direct RNA sequencing (DRS) enables measurements of RNA modifications. Modification-free transcripts are a practical and targeted control for DRS, providing a baseline measurement for canonical nucleotides within a matched and biologically derived sequence context. However, these controls can be challenging to generate and carry nanopore-specific nuances that can impact analysis. We produced DRS datasets using modification-free transcripts from in vitro transcription (IVT) of cDNA from six immortalized human cell lines. We characterized variation across cell lines and demonstrated how these may be interpreted. These data will serve as a versatile control and resource to the community for RNA modification analysis of human transcripts.

### Competing Interest Statement

The authors have declared no competing interest.

*   DRS
    :   direct RNA sequencing
    
    IVT
    :   in vitro transcription
    
    Ψ
    :   pseudouridine
    
    m6A
    :   N 6-methyladenosine
    
    I
    :   inosine
    
    poly-A
    :   polyadenylated
    
    gDNA
    :   genomic DNA
    
    panIVT
    :   pooled aligned IVT reads
    
    BED
    :   Browser Extensible Data
    
    IGV
    :   integrated genome viewing
    
    SNV
    :   single nucleotide variant

Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysis

인간-전사체의-수정되지-않은-다세포성-ivt-데이터셋을-이용한-나노포어-직접-rna-분석


인간 전사체의 수정되지 않은 다세포성 IVT 데이터셋을 이용한 나노포어 직접 RNA 분석
