
The domesticated transposon protein L1TD1 associates with its ancestral protein L1 ORF1p and promotes the retrotransposition of LINE-1 elements in human tumor cells.


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Repression of retrotransposition is crucial for the successful fitness of a mammalian organism. The domesticated transposon protein L1TD1, derived from LINE-1 ORF1p, is an RNA-binding protein that is expressed only in some cancers and early embryogenesis. In human embryonic stem cells it is found to be essential for maintaining pluripotency. In cancer, L1TD1 expression is highly correlative with malignancy progression and as such considered a potential prognostic factor for tumors. However, its molecular role in cancer remains largely unknown. Our findings reveal that DNA hypomethylation induces the expression of L1TD1 in HAP1 human tumor cells. L1TD1 depletion significantly modulates both the proteome and transcriptome and thereby reduces cell viability. Notably, L1TD1 associates with LINE-1 transcripts and interacts with LINE-1 ORF1p protein, thereby facilitating LINE-1 retrotransposition. Our data suggest that L1TD1 collaborates with its ancestral LINE-1 ORF1p as an RNA chaperone, ensuring the efficient retrotransposition of LINE-1 retrotransposons, rather than directly impacting the abundance of L1TD1 targets. In this way, L1TD1 might have an important role not only during early development but also in tumorigenesis.

### Competing Interest Statement

The authors have declared no competing interest.

The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

domesticated-transposon-protein-l1td1-promotes-line-1-retrotransposition-by-interacting-with-its-ancestral-protein-l1-orf1p


Domesticated Transposon Protein L1TD1 Promotes LINE-1 Retrotransposition by Interacting with its Ancestral Protein L1 ORF1p
