แนวคิดหลัก
AIRE relies on Z-DNA and NFE2–MAF to select gene targets for thymic T cell tolerization by enhancing double-stranded break generation and promoter poising.
บทคัดย่อ
AIRE, an unconventional transcription factor, influences gene expression in thymic cells to regulate self-reactive T cells. Through Z-DNA and NFE2–MAF, AIRE targets genes with poised promoters, shedding light on mechanisms of T cell tolerance manipulation.
สถิติ
AIRE enhances the expression of thousands of genes in medullary thymic epithelial cells.
Z-DNA and NFE2–MAF are identified as positive influences on AIRE’s target choices.
Genome-wide mapping studies show that Z-DNA-forming motifs are associated with DNA double-stranded break generation in gene promoters.
Promoters with strong double-stranded break generation are more likely to have poised states with accessible chromatin.
AIRE preferentially targets genes with poised promoters.
คำพูด
"We propose a model in which Z-DNA anchors the AIRE-mediated transcriptional program by enhancing double-stranded break generation and promoter poising."