核心概念
The IDA peptide signaling pathway regulates both developmental cell separation processes and immune responses in Arabidopsis, ensuring optimal protection of cells undergoing separation that are vulnerable to pathogen attack.
摘要
The content explores the role of the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide signaling pathway in coordinating cell separation processes and immune responses in Arabidopsis. Key highlights:
- IDA induces the production of reactive oxygen species (ROS) and an increase in cytosolic calcium (Ca2+) levels, which are signatures of early defense responses.
- IDA also promotes the transcriptional upregulation of defense-associated genes, indicating its involvement in late immune responses.
- The IDA-induced Ca2+ and ROS responses show both similarities and differences compared to the well-studied flg22-triggered immune responses, suggesting specific signaling components are involved.
- IDA expression is upregulated by biotic (e.g., flg22, chitin) and abiotic (e.g., salt, osmotic stress) stimuli, indicating its role in coordinating cell separation and defense.
- Co-treatment with IDA and the immune elicitor flg22 leads to a synergistic enhancement of defense gene expression, suggesting IDA helps amplify immune responses in cells undergoing separation.
- The receptors HAE and HSL2 that mediate IDA signaling show overlapping expression with the immune receptor FLS2 in cells involved in cell separation processes, providing a potential mechanism for the coordination of development and defense.
The authors propose a model where IDA signaling ensures optimal immune responses in cells destined for separation, as these cells are major entry points for pathogens during the cell wall remodeling process.
統計資料
Transcripts of FRK1, MYB51, and PEP3 were significantly increased in Arabidopsis seedlings treated with 1 μM mIDA for 1 hour compared to untreated controls.
Co-treatment with 1 μM mIDA and 1 μM flg22 for 1 hour led to a synergistic enhancement of FRK1, MYB51, and PEP3 transcription compared to treatment with either peptide alone.
引述
"IDA induces a release of cytosolic calcium ions (Ca2+) and apoplastic production of reactive oxygen species, which are signatures of early defense responses."
"IDA promotes late defense responses by the transcriptional upregulation of genes known to be involved in immunity."
"We propose a molecular mechanism by which IDA promotes signatures of an immune response in cells destined for separation to guard them from pathogen attack."