
확장 지원 선택적 평면 조명 현미경(ExA-SPIM)은 센티미터 규모의 조직을 나노스케일 해상도로 이미징할 수 있는 새로운 현미경 기술이다.


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Recent advances in tissue processing, labeling, and fluorescence microscopy are providing unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and near single molecule sensitivity, driving discoveries in diverse fields of biology, including neuroscience. Biological tissue is organized over scales of nanometers to centimeters. Harnessing molecular imaging across intact, three-dimensional samples on this scale requires new types of microscopes with larger fields of view and working distance, as well as higher throughput. We present a new expansion-assisted selective plane illumination microscope (ExA-SPIM) with aberration-free 1×1×3 μm optical resolution over a large field of view (10.6×8.0 mm2) and working distance (35 mm) at speeds up to 946 megavoxels/sec. Combined with new tissue clearing and expansion methods, the microscope allows imaging centimeter-scale samples with 250×250×750 nm optical resolution (4× expansion), including entire mouse brains, with high contrast and without sectioning. We illustrate ExA-SPIM by reconstructing individual neurons across the mouse brain, imaging cortico-spinal neurons in the macaque motor cortex, and visualizing axons in human white matter.

### Competing Interest Statement

A.G., J.C., and K.S. have filed for a patent on aspects of the ExA-SPIM system.

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues

확장-지원-선택적-평면-조명-현미경을-이용한-센티미터-규모-조직의-나노스케일-이미징


확장 지원 선택적 평면 조명 현미경을 이용한 센티미터 규모 조직의 나노스케일 이미징
