
IRE1シグナリングは、プロリン4-ヒドロキシラーゼ(P4HB)の発現を調節することで、コラーゲン生合成と分泌を制御し、肝臓疾患の病態形成に重要な役割を果たす。


coremsg

Collagen is one the most abundant proteins and the main cargo of the secretory pathway, contributing to hepatic fibrosis and cirrhosis due to excessive deposition of extracellular matrix. Here we investigated the possible contribution of the unfolded protein response, the main adaptive pathway that monitors and adjusts the protein production capacity at the endoplasmic reticulum, to collagen biogenesis and liver disease. Genetic ablation of the ER stress sensor IRE1 reduced liver damage and diminished collagen deposition in models of liver fibrosis triggered by carbon tetrachloride (CCl4) administration or by high fat diet. Proteomic and transcriptomic profiling identified the prolyl 4-hydroxylase (P4HB, also known as PDIA1), which is known to be critical for collagen maturation, as a major IRE1-induced gene. Cell culture studies demonstrated that IRE1 deficiency results in collagen retention at the ER and altered secretion, a phenotype rescued by P4HB overexpression. Taken together, our results collectively establish a role of the IRE1/P4HB axis in the regulation of collagen production and its significance in the pathogenesis of various disease states.

### Competing Interest Statement

The authors have declared no competing interest.

The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis

肝臓の内皮小胞体ストレスセンサーire1は-p4hb-pdia1プロテオスタシス因子の強化を通じて-コラーゲン分泌を調節し-肝臓損傷と線維症に寄与する


肝臓の内皮小胞体ストレスセンサーIRE1は、P4HB/PDIA1プロテオスタシス因子の強化を通じて、コラーゲン分泌を調節し、肝臓損傷と線維症に寄与する
