
의료 진단 회사와 생물다양성 연구소의 협력을 통해 1주일 내에 휘펫 개의 고품질 염색체 수준 유전체를 신속하게 조립하고 주석을 작성하였다.


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Background The time required for sequencing and de novo assembly of genomes is highly dependent on the interaction between laboratory work, sequencing capacity, and the bioinformatics workflow. As a result, genome projects are often not only limited by financial, computational and sequencing platform resources, but also delayed by second party sequencing service providers. By bringing together academic biodiversity institutes and a medical diagnostics company with extensive sequencing capabilities and know-how, we aimed at generating a high-quality mammalian de novo genome in the shortest possible time period. Therefore, we streamlined all processes involved and chose a very fast dog as a model: The Whippet.

Findings We present the first chromosome-level genome assembly of the Whippet. We used PacBio long-read HiFi sequencing and reference-guided scaffolding to generate a high-quality genome assembly. The final assembly has a contig N50 of 55 Mbp and a scaffold N50 of 65.7 Mbp. The total assembly length is 2.47 Gbp, of which 2.43 Gpb were scaffolded into 39 chromosome-length scaffolds. In addition, we used available mammalian genomes and transcriptome data to annotate the genome assembly. The annotation resulted in 28,383 transcripts resembling a total of 90.9% complete BUSCO genes and identified a repeat content of 36.5%.

Conclusions Sequencing, assembling, and scaffolding the chromosome-level genome of the Whippet took less than a week and adds a high-quality reference genome to the list of domestic dog breeds sequenced to date.

### Competing Interest Statement

The authors have declared no competing interest.

*   BUSCO
    :   Benchmarking Universal Single-Copy Orthologs
    CCS
    :   Circular Consensus Sequencing
    HiFi
    :   High Fidelity
    LINEs
    :   Long interspersed nuclear elements
    LR-WGS
    :   Long-Read Whole-Genome Sequencing
    LTR
    :   Long Terminal Repeat
    SFS
    :   Site Frequency Spectrum

High-speed whole-genome sequencing of a Whippet: Rapid chromosome-level assembly and annotation of an extremely fast dog’s genome

초고속-전장-유전체-염기서열-분석을-통한-휘펫-개의-염색체-수준-유전체-조립-및-주석-작성


초고속 전장 유전체 염기서열 분석을 통한 휘펫 개의 염색체 수준 유전체 조립 및 주석 작성
