
The generation of a transgenic Lifeact-EGFP quail line enables high-resolution live imaging of actin structures and dynamics during key morphogenetic processes in the developing avian embryo.


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Here we report the generation of a transgenic Lifeact–EGFP quail line for the investigation of actin organization and dynamics during morphogenesis in vivo . This transgenic avian line allows for the high-resolution visualization of actin structures within the living embryo, from the subcellular filaments that guide cell shape to the supracellular assemblies that coordinate movements across tissues. The unique suitability of avian embryos to live imaging facilitates the investigation of previously intractable processes during embryogenesis. Using high-resolution live imaging approaches, we present the dynamic behaviours and morphologies of cellular protrusions in different tissue contexts. Furthermore, through the integration of live imaging with computational segmentation, we reveal the dynamics of cells undergoing apical constriction and the emergence of large-scale actin structures such as supracellular cables and rosettes within the neuroepithelium. These findings not only enhance our understanding of tissue morphogenesis but also demonstrate the utility of the Lifeact–EGFP transgenic quail as a new model system for live in vivo investigations of the actin cytoskeleton.

### Competing Interest Statement

The authors have declared no competing interest.

The Lifeact-EGFP Quail: A New Avian Model For Studying Actin Dynamics In Vivo

the-lifeact-egfp-quail-a-powerful-avian-model-for-live-imaging-of-actin-dynamics-during-embryonic-morphogenesis


The Lifeact-EGFP Quail: A Powerful Avian Model for Live Imaging of Actin Dynamics During Embryonic Morphogenesis
