
The authors have developed a series of genetically engineered mouse lines that enable specific and comprehensive targeting of claustrum neurons, providing new tools to study the functions of this enigmatic brain structure.


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The claustrum (CLA), a subcortical nucleus in mammals, essentially composed of excitatory projection neurons and known for its extensive connections with the neocortex, has recently been associated with a variety of functions ranging from consciousness to impulse control. However, research on the CLA has been challenging due to difficulties in specifically and comprehensively targeting its neuronal populations. In various cases, this limitation has led to inconsistent findings and a lack of reliable data. In the present work, we describe the expression profile of the Smim32 gene, which is almost exclusively transcribed in excitatory neurons of the CLA and the endopiriform nucleus, as well as in inhibitory neurons of the thalamic reticular nucleus. Leveraging this unique expression pattern, we developed a series of Cre- and Flippase-expressing knockin and BAC transgenic mouse lines with different expression profiles. With these novel tools in hand, we propose new standards for the interrogation of CLA function.

### Competing Interest Statement

The authors have declared no competing interest.

A toolbox for genetic targeting of the claustrum

a-comprehensive-toolbox-for-genetic-targeting-of-the-claustrum-in-mice


A Comprehensive Toolbox for Genetic Targeting of the Claustrum in Mice
