Khái niệm cốt lõi
Deciphering the mechanism of bacterial gasdermin pore formation reveals diverse pore sizes and an ancient post-translational modification enabling programmed host cell death.
Tóm tắt
The content delves into the structure and assembly of bacterial gasdermin (bGSDM) pores in response to pathogen infection, leading to pyroptosis. Key highlights include:
- Human and mouse GSDM pores consist of 24 to 33 protomers.
- The mechanism and evolutionary origin of membrane targeting and GSDM pore formation are unknown.
- A study on bGSDMs shows diverse pore sizes ranging from mammalian-like assemblies to large pores with over 50 protomers.
- A cryo-electron microscopy structure of a Vitiosangium bGSDM in an active oligomeric conformation is determined.
- Molecular dynamics simulations and cellular assays support a stepwise model of GSDM pore assembly.
Thống kê
Studies reveal human and mouse GSDM pores comprise 24 to 33 protomers.
Diverse bGSDMs form distinct pore sizes ranging from smaller mammalian-like assemblies to exceptionally large pores containing more than 50 protomers.