The study investigates how bacteria isolated from the natural environment of the nematode Caenorhabditis elegans affect the transmission and infection of the Orsay virus, a naturally occurring virus that infects C. elegans.
The key findings are:
Ochrobactrum species promote Orsay virus transmission, while Pseudomonas lurida MYb11 and Pseudomonas aeruginosa strains PA01 and PA14 attenuate virus transmission.
The presence of Ochrobactrum vermis MYb71 reduces the amount of Orsay virus required to infect 50% of the C. elegans population by over three orders of magnitude compared to P. lurida MYb11. P. aeruginosa PA14 further attenuates host susceptibility.
Genetic analysis reveals that the attenuation of Orsay virus transmission and infection by P. aeruginosa and P. lurida is dependent on bacterial quorum sensing regulators like gacA, as well as other virulence-related genes.
Mutation of the gacA regulator in P. lurida MYb11 suppresses the attenuation of Orsay virus transmission and infection, suggesting a conserved role for quorum sensing in modulating virus-host interactions across different bacterial species.
Electron microscopy suggests that Ochrobactrum vermis MYb71 may disrupt the intestinal brush border and glycocalyx of C. elegans, potentially promoting viral infection.
Overall, the study provides quantitative evidence for the critical role of tripartite host-virus-bacteria interactions in determining viral transmission and infection rates.
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by Vassallo,B. ... lúc www.biorxiv.org 09-05-2023
https://www.biorxiv.org/content/10.1101/2023.09.05.556377v2Yêu cầu sâu hơn