Core Concepts
Loss of the transcription factor GPR156 prevents the reversal of hair cell orientation in mouse otolith organs and zebrafish lateral line, but does not significantly impact hair cell physiology, afferent innervation patterns, or overall vestibular function.
Abstract
The content explores how the molecular mechanisms that confer bidirectional hair cell orientation in the vestibular system and lateral line contribute to sensory function. Key findings:
In mouse otolith organs, loss of GPR156 prevents the reversal of hair cell orientation in the lateral extrastriolar (LES) region, but does not affect hair cell numbers, zonal organization, or mechanotransduction properties.
Afferent innervation patterns and selectivity for hair cells of opposing orientations were preserved in the mouse utricle lacking GPR156, despite the absence of the line of polarity reversal. Afferent excitability was also unaffected.
While GPR156 deletion did not impact mouse vestibular function, performance on tests engaging otolith organs was altered, suggesting the line of polarity reversal contributes to specific aspects of vestibular processing.
In the zebrafish lateral line, loss of GPR156 resulted in all hair cells adopting the orientation that detects anterior flow. Importantly, this led to a reduction in the mechanosensitive responses of these hair cells compared to the larger responses of the anterior-detecting hair cells in control animals.
Afferent innervation patterns and synaptic pairing were preserved in zebrafish lateral line lacking GPR156, despite the absence of hair cell orientation reversal.
The results clarify how the molecular mechanisms underlying hair cell orientation reversal contribute to sensory organ function, from single cell physiology to animal behavior, in both the vestibular system and lateral line.
Stats
The maximum conductance density (Gmax/Cm) of the voltage-gated K+ currents in type I hair cells was much larger than in type II hair cells.
The activation midpoint (V1/2) of the voltage-gated outward currents in type II hair cells was around -30 to -40 mV.
The maximum GMET of type I hair cells was larger in the medial extrastriolar (MES) zone compared to the LES or striolar zones.
Quotes
"Loss of GPR156 does not impact HC orientation in the striola and MES, but Gpr156 is transcribed in all HCs across the maculae."
"Afferent innervation patterns and selectivity for hair cells of opposing orientations were preserved in the mouse utricle lacking GPR156, despite the absence of the line of polarity reversal."
"In the zebrafish lateral line, loss of GPR156 resulted in all hair cells adopting the orientation that detects anterior flow. Importantly, this led to a reduction in the mechanosensitive responses of these hair cells compared to the larger responses of the anterior-detecting hair cells in control animals."