
プラスミド由来の免疫グロブリン様ドメインを含むタンパク質は抗原性が高く、これらをターゲットにすることで抗菌薬耐性感染症に対する防御効果が得られる。


coremsg

Antimicrobial resistance (AMR) poses a significant threat to human health. Although vaccines have been developed to combat AMR, it has proven challenging to associate specific vaccine antigens with AMR. Bacterial plasmids play a crucial role in the transmission of AMR. Our recent research has identified a group of bacterial plasmids (specifically, IncHI plasmids) that encode large molecular mass proteins containing bacterial immunoglobulin-like domains. These proteins are found on the external surface of the bacterial cells, such as in the flagella or conjugative pili. In this study, we show that these proteins are antigenic and can protect mice from infection caused by an AMR Salmonella strain harboring one of these plasmids. Furthermore, we successfully generated nanobodies targeting these proteins, that were shown to interfere with the conjugative transfer of IncHI plasmids. Considering that these proteins are also encoded in other groups of plasmids, such as IncA/C and IncP2, targeting them could be a valuable strategy in combating AMR infections caused by bacteria harboring different groups of AMR plasmids. Since the selected antigens are directly linked to AMR itself, the protective effect extends beyond specific microorganisms to include all those carrying the corresponding resistance plasmids.

### Competing Interest Statement

The authors have declared no competing interest.

Targeting plasmid-encoded proteins that contain immunoglobulin-like domains to combat antimicrobial resistance

抗菌薬耐性に対処するための免疫グロブリン様ドメインを含むプラスミド由来タンパク質のターゲティング