
세포 단층에서 핵-세포질 수송은 세포 밀도와 무관하게 기계적 자극에 의해 조절된다.


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Cells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health and disease. In single cells, mechanotransduction involves the transmission of force to the cell nucleus, where it affects nucleocytoplasmic transport (NCT) and the subsequent nuclear localization of transcriptional regulators such as YAP. However, if and how NCT is mechanosensitive in multicellular systems is unclear. Here, we characterize and use a fluorescent sensor of nucleocytoplasmic transport (Sencyt) and demonstrate that nucleocytoplasmic transport responds to mechanics but not cell density in cell monolayers. Using monolayers of both epithelial and mesenchymal phenotype, we show that NCT is altered in response both to osmotic shocks, and to the inhibition of cell contractility. Further, NCT correlates with the degree of nuclear deformation measured through nuclear solidity, a shape parameter related to nuclear envelope tension. In contrast and in opposition to YAP, NCT is not affected by cell density, showing that the response of YAP to both mechanics and cell-cell contacts operates through distinct mechanisms. Our results demonstrate the generality of the mechanical regulation of NCT.

Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers

세포-단층에서-세포-밀도와-무관하게-핵-세포질-수송이-기계적-자극을-감지한다-


세포 단층에서 세포 밀도와 무관하게 핵-세포질 수송이 기계적 자극을 감지한다.
