
NAA80 효소는 제브라피쉬에서 액틴의 N 말단 아세틸화에 필수적이며, 이러한 변형은 제브라피쉬의 정상적인 청력 발달에 중요한 역할을 한다.


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Actin is a key component of the cytoskeleton of eukaryotic cells and is involved in numerous cellular functions. In animal cells, actins are uniquely N-terminally processed by a dedicated enzyme machinery to generate their mature acidic and acetylated forms. The final step of this maturation process involves N-terminal acetylation, a reaction catalyzed by NAA80 in humans. In human cell lines, N-terminal acetylation of actin plays a crucial role in maintaining normal cytoskeletal dynamics and cell motility. The physiological impact of actin N-terminal acetylation remains to be defined. Here, we developed a zebrafish naa80 knockout model and established that zNaa80 acetylates both muscle and non-muscle actins in vivo . Our in vitro investigation of purified zNaa80 unveiled a clear preference for acetylating N-termini derived from actins. Interestingly, zebrafish lacking actin N-terminal acetylation were viable and exhibited normal development, morphology and behaviour. In contrast, human individuals carrying pathogenic actin variants may present with hypotonia and hearing impairment. While zebrafish depleted for naa80 did not display any obvious muscle defects or abnormal muscle tissue, we found that they have abnormal inner ear development such as small otoliths and impaired response to sound stimuli. In sum, we have defined that zebrafish Naa80 N-terminally acetylates actins in vitro and in vivo and that actin N-terminal acetylation is essential for normal hearing in vivo .

### Competing Interest Statement

The authors have declared no competing interest.

Naa80 is required for actin N-terminal acetylation and normal hearing in zebrafish

제브라피쉬에서-naa80는-액틴-n-말단-아세틸화-및-정상-청력에-필수적이다


제브라피쉬에서 NAA80는 액틴 N 말단 아세틸화 및 정상 청력에 필수적이다
