
DIETS 기법을 통해 초파리의 고체 식이 섭취량을 직접 측정하고 장기간 추적할 수 있으며, 이를 통해 다양한 식이 조건에 따른 초파리의 생리적 변화를 연구할 수 있다.


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The fruit fly Drosophila melanogaste r offers a powerful model to study how diet affects the body and brain. However, existing methods for measuring their food intake often rely on dyes or tags mixed with food, which can be inaccurate due to how the flies absorb and eliminate them. Capillary-based assays like CAFE directly measure consumption, but only work with liquids and shorten fly lifespan. Additionally, capillary assays are incompatible with delivering viscous foods like high-fat diets. Even solidified high-fat diets tend to be sticky death traps for flies. Another longstanding challenge for fly researchers is that dietary restriction in flies involves diluting food, leading to compensatory feeding. To address these shortcomings, we have developed DIETS, a sensitive feeding assay that can be implemented even in low-resource settings. DIETS eliminates the need for labels and directly weighs the solid food consumed by small groups of flies over extended periods of hours to weeks. It allows us to deliver precise amounts of food to flies and implement accurate dietary restrictions. Importantly, DIETS is compatible with studying energy-dense high-fat diets. Using DIETS, we observed that, unlike a high-sugar diet, an isocaloric high-fat diet did not improve the flies’ ability to withstand starvation, even though they consumed more calories and had higher fat deposition.

### Competing Interest Statement

The authors have declared no competing interest.

DIETS: a simple and sensitive assay to measure and control the intake of complex solid foods, like high-fat diets, in Drosophila

고체-식이-섭취량-직접-측정-및-장기-추적-diets-초파리에서의-활용


고체 식이 섭취량 직접 측정 및 장기 추적(DIETS): 초파리에서의 활용
