
Repeated activation of negative memory engrams in the ventral hippocampus of mice induces lasting anxiety-like behaviors, impairs spatial working memory, and disrupts fear extinction and generalization, alongside cellular changes in microglia, astrocytes, and GABAergic signaling.


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Negative memories engage a brain and body-wide stress response in humans that can alter cognition and behavior. Prolonged stress responses induce maladaptive cellular, circuit, and systems-level changes that can lead to pathological brain states and corresponding disorders in which mood and memory are affected. However, it’s unclear if repeated activation of cells processing negative memories induces similar phenotypes in mice. In this study, we used an activity-dependent tagging method to access neuronal ensembles and assess their molecular characteristics. Sequencing memory engrams in mice revealed that positive (male-to-female exposure) and negative (foot shock) cells upregulated genes linked to anti- and pro-inflammatory responses, respectively. To investigate the impact of persistent activation of negative engrams, we chemogenetically activated them in the ventral hippocampus over three months and conducted anxiety and memory-related tests. Negative engram activation increased anxiety behaviors in both 6- and 14-month-old mice, reduced spatial working memory in older mice, impaired fear extinction in younger mice, and heightened fear generalization in both age groups. Immunohistochemistry revealed changes in microglia and astrocytes, alongside GABAergic fluorescence reduction in the hippocampus. In summary, repeated activation of negative memories induces lasting cellular and behavioral abnormalities in mice, offering insights into the negative effects of rumination on human health.

### Competing Interest Statement

The authors have declared no competing interest.

Chronic activation of a negative engram induces behavioral and cellular abnormalities

chronic-activation-of-negative-memory-engrams-induces-lasting-behavioral-and-cellular-abnormalities-in-mice


Chronic Activation of Negative Memory Engrams Induces Lasting Behavioral and Cellular Abnormalities in Mice
