Structural Characterization of pH-Dependent Enzymatic Activity and Ligand Binding of Mouse Acidic Mammalian Chitinase
Mouse Acidic Mammalian Chitinase (mAMCase) exhibits dual pH optima at pH 2.0 and 6.5, allowing it to function in both acidic and neutral environments. Structural, biochemical, and computational analyses reveal how mAMCase employs distinct mechanisms to protonate its catalytic glutamate residue depending on the environmental pH.