
CD9과 CD81 테트라스파닌은 터널링 나노튜브 형성과 기능에 있어 서로 다른 비중복적인 역할을 한다. CD9은 나노튜브의 안정화에 기여하고, CD81은 나노튜브를 통한 소포 전달에 필요하다.


coremsg

Tunneling nanotubes (TNTs) are open actin- and membrane-based channels, connecting remote cells and allowing direct transfer of cellular material (e.g. vesicles, mRNAs, protein aggregates) from cytoplasm to cytoplasm. Although they are important especially in pathological conditions (e.g., cancers, neurodegenerative diseases), their precise composition and their regulation were still poorly described. Here, using a biochemical approach allowing to separate TNTs from cell bodies and from extracellular vesicles and particles (EVPs), we obtained the full composition of TNTs compared to EVPs. We then focused to two major components of our proteomic data, the CD9 and CD81 tetraspanins, and further investigated their specific roles in TNT formation and function. We show that these two tetraspanins have distinct non-redundant functions: CD9 participates in stabilizing TNTs, whereas CD81 expression is required to allow the functional transfer of vesicle in the newly formed TNTs, possibly by regulating docking to or fusion with the opposing cell.

### Competing Interest Statement

The authors have declared no competing interest.

Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

터널링-나노튜브의-프로테오믹-분석을-통해-cd9과-cd81-테트라스파닌이-핵심-조절자임이-밝혀짐


터널링 나노튜브의 프로테오믹 분석을 통해 CD9과 CD81 테트라스파닌이 핵심 조절자임이 밝혀짐
