
The E3 ubiquitin ligase Deltex (Dx) regulates Wingless (Wg) signaling in Drosophila by facilitating the spreading of the Wg gradient and promoting the proteasomal degradation of the Wg effector Armadillo/β-catenin.


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The Wnt/Wg pathway controls myriads of biological phenomena throughout the development and adult life of all organisms across the phyla. Thus, an aberrant Wnt signaling is associated with a wide range of pathologies in humans. Tight regulation of Wnt/Wg signaling is required to maintain proper cellular homeostasis. Here we report a novel role of E3 ubiquitin ligase Deltex in Wg signaling regulation. Drosophila dx genetically interacts with wg a nd its pathway components. Further, Dx LOF results in a reduced spreading of Wg while its over-expression expands the diffusion gradient of the morphogen. We attribute this change in Wg gradient to the endocytosis of Wg through Dx which directly affects the short and long-range Wg targets. We also demonstrate the role of Dx in regulating Wg effector Armadillo where Dx down-regulates Arm through proteasomal degradation. We also showed the conservation of Dx function in the mammalian system where DTX1 is shown to bind with β-catenin and facilitates its proteolytic degradation, spotlighting a novel step that potentially modulates Wnt/Wg signaling cascade.

### Competing Interest Statement

The authors have declared no competing interest.

E3 ubiquitin ligase Deltex facilitates the expansion of Wingless gradient and antagonizes Wingless signaling through a conserved mechanism of transcriptional effector Armadillo/β-catenin degradation

the-e3-ubiquitin-ligase-deltex-regulates-wingless-gradient-formation-and-signaling-through-armadillo-β-catenin-degradation


The E3 Ubiquitin Ligase Deltex Regulates Wingless Gradient Formation and Signaling through Armadillo/β-Catenin Degradation
