hochwertige-und-flexible-szenenrekonstruktion-aus-uneingeschränkten-bildsammlungen-durch-3d-gaussian-splatting

다중-객체-추적을-위한-파레토-신경-구조-탐색

identifizierbare-latente-neuronale-kausale-modelle

제로샷-스탠스-탐지를-위한-인코더-디코더-데이터-증강-프레임워크

다중-모바일-로봇의-생체모방-신경-동역학-기반-분산-강인-학습-형성-제어

effiziente-und-robuste-mehrfachobjektverfolgung-durch-pareto-suche-neuronaler-architekturen

과학-컴퓨팅을-위한-이기종-데이터-파이프라인-radical-cylon

eine-encoder-decoder-datenausgaberahmenwerk-für-zero-shot-haltungserkennung


BMP シグナル活性化により引き起こされる羊膜分化の時間依存的な転写カスケードを明らかにした。TFAP2A が羊膜分化の進行に重要な役割を果たすことを示した。


coremsg

Amniogenesis, a process critical for continuation of healthy pregnancy, is triggered in a collection of pluripotent epiblast cells as the human embryo implants. Previous studies have established that BMP signaling is a major driver of this lineage specifying process, but the downstream BMP-dependent transcriptional networks that lead to successful amniogenesis remain to be identified. This is, in part, due to the current lack of a robust and reproducible model system that enables mechanistic investigations exclusively into amniogenesis. Here, we developed an improved model of early amnion specification, using a human pluripotent stem cell-based platform in which the activation of BMP signaling is controlled and synchronous. Uniform amniogenesis is seen within 48 hours after BMP activation, and the resulting cells share transcriptomic characteristics with amnion cells of a gastrulating human embryo. Using detailed time-course transcriptomic analyses, we established a previously uncharacterized BMP-dependent amniotic transcriptional cascade, and identified markers that represent five distinct stages of amnion fate specification; the expression of selected markers was validated in early post-implantation macaque embryos. Moreover, a cohort of factors that could potentially control specific stages of amniogenesis was identified, including the transcription factor TFAP2A. Functionally, we determined that, once amniogenesis is triggered by the BMP pathway, TFAP2A controls the progression of amniogenesis. This work presents a temporally resolved transcriptomic resource for several previously uncharacterized amniogenesis states and demonstrates a critical intermediate role for TFAP2A during amnion fate specification.

### Competing Interest Statement

The authors have declared no competing interest.

Temporally resolved early BMP-driven transcriptional cascade during human amnion specification

ヒト羊膜の初期分化過程における時間依存的な-bmp-誘導性転写カスケードの解明


ヒト羊膜の初期分化過程における時間依存的な BMP 誘導性転写カスケードの解明


title_rewrite


シングナシデ科の特徴的な形質(頭部形状、無歯、皮膚骨格、雄の育児)の発生遺伝学的基盤を明らかにした。


Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, and dermal bony armor. The developmental genetic and cellular changes that led to the evolution of these traits are largely unknown. Recent syngnathid genomes revealed suggestive gene content differences and provide the opportunity for detailed genetic analyses. We created a single cell RNA sequencing atlas of Gulf pipefish embryos to understand the developmental basis of four traits: derived head shape, toothlessness, dermal armor, and male pregnancy. We completed marker gene analyses, built genetic networks, and examined spatial expression of select genes. We identified osteochondrogenic mesenchymal cells in the elongating face that express regulatory genes bmp4, sfrp1a , and prdm16 . We found no evidence for tooth primordia cells, and we observed re-deployment of osteoblast genetic networks in developing dermal armor.

Finally, we found that epidermal cells expressed nutrient processing and environmental sensing genes, potentially relevant for the brooding environment. The examined pipefish evolutionary innovations are composed of recognizable cell types, suggesting derived features originate from changes within existing gene networks. Future work addressing syngnathid gene networks across multiple stages and species is essential for understanding how their novelties evolved.

Impact Statement The production of a single cell atlas for developing syngnathid fish permits study of their unique traits.

### Competing Interest Statement

The authors have declared no competing interest.

Single Cell RNA Sequencing Provides Clues for the Developmental Genetic Basis of Syngnathidae’s Evolutionary Adaptations

単一細胞rna配列解析は-シングナシデ科の進化的適応の発生遺伝学的基盤に関するヒントを提供する


単一細胞RNA配列解析は、シングナシデ科の進化的適応の発生遺伝学的基盤に関するヒントを提供する



体軸分節化の時間的制御と空間的制御は独立したモジュールであり、自然環境下では選択圧によって結合されている。


How temporal and spatial control of developmental processes are linked remains a fundamental question. Do underlying mechanisms form a single functional unit or are these dissociable modules?

We address this question by studying the periodic process of embryonic axis segmentation, using genetic crosses of inbred medaka fish strains representing two species, Oryzias sakaizumii and latipes . Our analysis revealed correlated interspecies differences with regard to the timing of segmentation, the size of segments and of the presomitic mesoderm (PSM), from which segments are periodically formed. We then did interspecies crosses and real-time imaging quantifications, which revealed extensive phenotypic variation in ∼600 F2 embryos. Importantly, while the F2 analysis showed correlated changes of PSM and segment size, these spatial measures were not correlated to the timing of segmentation. This shows that the control of time and space of axis segmentation can, in principle, be decoupled. In line with this finding, we identified, using developmental quantitative trait loci ( dev QTL) mapping, distinct chromosomal regions linked to either the control of segmentation timing or PSM size. We were able to validate the dev QTL findings using a CRISPR/Cas9 loss-of-function approach on several candidate genes in vivo .

Combined, this study reveals that a developmental constraint mechanism underlies spatial scaling of axis segmentation, while its spatial and temporal control are dissociable modules. Our findings emphasise the need to reveal the selective constraints linking these modules in the natural environment.

### Competing Interest Statement

The authors have declared no competing interest.

Modular control of time and space during vertebrate axis segmentation

異なる脊椎動物種間における体軸分節化のタイミングと大きさの調節機構